| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The eBio1D3 monoclonal antibody has also been reported to induce down-regulation of CD19, affecting the proportions of B-1a and B-2 B cells in mice. (Please use Functional Grade purified eBio1D3 (1D3), cat. 16-0193, in functional assays.).Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 1. Nature immunology Postprandial macrophage-derived IL-1β stimulates insulin, and both synergistically promote glucose disposal and inflammation. "14019382 was used in flow cytometry to determine a physiological role for IL-1beta and insulin in the regulation of both metabolism and immunity" Authors Dror E,Dalmas E,Meier DT,Wueest S,Thévenet J,Thienel C,Timper K,Nordmann TM,Traub S,Schulze F,Item F,Vallois D,Pattou F,Kerr-Conte J,Lavallard V,Berney T,Thorens B,Konrad D,Böni-Schnetzler M,Donath MY2. PloS one Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice. "14019382 was used in flow cytometry to study the effect of IL-10 signaling in infectious neurological diseases" Authors Uhde AK,Herder V,Akram Khan M,Ciurkiewicz M,Schaudien D,Teich R,Floess S,Baumgärtner W,Huehn J,Beineke A3. PloS one Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice. "14019382 was used in flow cytometry to study the effect of IL-10 signaling in infectious neurological diseases" Authors Uhde AK,Herder V,Akram Khan M,Ciurkiewicz M,Schaudien D,Teich R,Floess S,Baumgärtner W,Huehn J,Beineke A4. Nature immunology Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment. "14019382 was used in flow cytometry to explore the contributions of GATA3 to the group 3 innate lymphoid cells" Authors Zhong C,Cui K,Wilhelm C,Hu G,Mao K,Belkaid Y,Zhao K,Zhu J5. Nature Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. "14019382 was used in flow cytometry to explain how glial cells impact ILC3-derived IL-22" Authors Ibiza S,García-Cassani B,Ribeiro H,Carvalho T,Almeida L,Marques R,Misic AM,Bartow-McKenney C,Larson DM,Pavan WJ,Eberl G,Grice EA,Veiga-Fernandes H |
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