| Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vitro functional studies. (Please use Functional Grade Purified HIB19, cat. 16-0199, in functional assays. Fluorochrome-conjugated HIB19 is recommended for use in flow cytometry.).Applications Tested: This HIB19 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.PloS oneThe Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.2017"14-0199 was used in Flow cytometry/Cell sorting to investigate the role of BDCA3+ DCs in immune response against the hepititis B virus, showing that BDCA3+ DCs are available in the blood and on site in HBV infected livers."Authors van der Aa E,Buschow SI,Biesta PJ,Janssen HL,Woltman AM2.OncotargetEfficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.2016"14-0199 was used in Flow cytometry/Cell sorting to expand human B-lymphocytes on a large scale while maintaining their antigen-presenting ability by using both CD40L and B-cell activating factor (BAFF)."Authors Yiwen Z,Shilin G,Yingshi C,Lishi S,Baohong L,Chao L,Linghua L,Ting P,Hui Z3.Journal of immunology (Baltimore, Md. : 1950)IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin.2016"14-0199 was used in Flow cytometry/Cell sorting to investigate innate-like B cells within the skin."Authors Geherin SA,Gómez D,Glabman RA,Ruthel G,Hamann A,Debes GF4.Journal of virologyThymic HIV-2 infection uncovers posttranscriptional control of viral replication in human thymocytes.2015"14-0199 was used in Flow cytometry/Cell sorting to assess the direct impact of HIV-2 infection on the human thymus."Authors Nunes-Cabaço H,Matoso P,Foxall RB,Tendeiro R,Pires AR,Carvalho T,Pinheiro AI,Soares RS,Sousa AE5.Rheumatology (Oxford, England)Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.2014"14-0199 was used in Flow cytometry/Cell sorting to study the frequencies of different B cell subtypes during progression of ANCA-associated vasculitis."Authors Todd SK,Pepper RJ,Draibe J,Tanna A,Pusey CD,Mauri C,Salama AD |
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