| Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.JCI insightEomesodermin and T-bet mark developmentally distinct human natural killer cells.2017"45-0199 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation."Authors Collins A,Rothman N,Liu K,Reiner SL2.PloS oneThe Phenotypic Characterization of the Human Renal Mononuclear Phagocytes Reveal a Co-Ordinated Response to Injury.2016"45-0199 was used in Flow cytometry/Cell sorting to analyse the characteristics and constitution of mononuclear phagocyte populations in human kidneys."Authors Leone DA,Kozakowski N,Kornauth C,Waidacher T,Neudert B,Loeffler AG,Haitel A,Rees AJ,Kain R3.The Journal of experimental medicineCX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.2014"45-0199 was used in Flow cytometry/Cell sorting to study the role of CX3CR1+ mononuclear phagocytes in the development of colitis and disease progression following Citrobacter rodentium infection."Authors Longman RS,Diehl GE,Victorio DA,Huh JR,Galan C,Miraldi ER,Swaminath A,Bonneau R,Scherl EJ,Littman DR4.Nature communicationsBiallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity."45-0199 was used in Flow cytometry/Cell sorting to study the effect of a biallelic mutation in the gene encoding NF-κB-inducing kinase on immune cell populations."Authors Willmann KL,Klaver S,Doğu F,Santos-Valente E,Garncarz W,Bilic I,Mace E,Salzer E,Conde CD,Sic H,Májek P,Banerjee PP,Vladimer GI,Haskoloğlu S,Bolkent MG,Küpesiz A,Condino-Neto A,Colinge J,Superti-Furga G,Pickl WF,van Zelm MC,Eibel H,Orange JS,Ikincioğulları A,Boztuğ K |
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