Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: This HIB19 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of human mononuclear cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. |
| 1.JCI insightEomesodermin and T-bet mark developmentally distinct human natural killer cells.2017"48-0199 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation."Authors Collins A,Rothman N,Liu K,Reiner SL2.PLoS pathogensCytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.2016"48-0199 was used in Flow cytometry/Cell sorting to reveal the marked accumulation and unique phenotype of CMV-specific CD4+ T cells."Authors Pachnio A,Ciaurriz M,Begum J,Lal N,Zuo J,Beggs A,Moss P3.PloS onePhenotypic, genomic and functional characterization reveals no differences between CD138++ and CD138low subpopulations in multiple myeloma cell lines.2015"48-0199 was used in Flow cytometry/Cell sorting to investigate the phenotype of multiple myeloma cancer stem cells, showing no differences between CD138++ and CD138low subpopulations."Authors Paíno T,Sarasquete ME,Paiva B,Krzeminski P,San-Segundo L,Corchete LA,Redondo A,Garayoa M,García-Sanz R,Gutiérrez NC,Ocio EM,San-Miguel JF4.Molecular therapy : the journal of the American Society of Gene TherapyCombining viral vectored and protein-in-adjuvant vaccines against the blood-stage malaria antigen AMA1: report on a phase 1a clinical trial."48-0199 was used in Flow cytometry/Cell sorting to identify new subunit vaccination strategies that can induce and maintain effective immune responses in humans."2014Authors Hodgson SH,Choudhary P,Elias SC,Milne KH,Rampling TW,Biswas S,Poulton ID,Miura K,Douglas AD,Alanine DG,Illingworth JJ,de Cassan SC,Zhu D,Nicosia A,Long CA,Moyle S,Berrie E,Lawrie AM,Wu Y,Ellis RD,Hill AV,Draper SJ5.Rheumatology (Oxford, England)Altered B cell balance, but unaffected B cell capacity to limit monocyte activation in anti-neutrophil cytoplasmic antibody-associated vasculitis in remission.2014"48-0199 was used in Flow cytometry/Cell sorting to assess the frequency and function of Bregs in patients with ANCA-associated vasculitis."Authors Lepse N,Abdulahad WH,Rutgers A,Kallenberg CG,Stegeman CA,Heeringa P |
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