ANTI-HUMAN CD45 (HI30) PE

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ANTI-HUMAN CD45 (HI30) PE

货号:12-0459-42,12-0459-41

规格:100T,25T

价格:753,909

产品类型:流式抗体

品牌:eBioscience

抗原:CD45

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:HI30

荧光染料:PE

抗体类型:单抗同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE
免疫原性:用法:5uL(0.06µg)/test(Flow)
Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD45 Antibody (12-0459-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD45 PE (purple histogram). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.Genes and developmentUTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.2016"12-0459 was used in Flow cytometry/Cell sorting to identify a subtype-specific epigenetic vulnerability in T-cell acute lymphoblastic leukaemia which is uniquely sensitive to a histone demethylase."Authors Benyoucef A,Palii CG,Wang C,Porter CJ,Chu A,Dai F,Tremblay V,Rakopoulos P,Singh K,Huang S,Pflumio F,Hébert J,Couture JF,Perkins TJ,Ge K,Dilworth FJ,Brand M2.Scientific reportsPotency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing.2016"12-0459 was used in Flow cytometry/Cell sorting to determine that umbilical cord blood offers a potential source of MSCs for therapeutic scarless wound healing."Authors Doi H,Kitajima Y,Luo L,Yan C,Tateishi S,Ono Y,Urata Y,Goto S,Mori R,Masuzaki H,Shimokawa I,Hirano A,Li TS3.HaematologicaPharmacological targeting of β-catenin in normal karyotype acute myeloid leukemia blasts.2015"12-0459 was used in Flow cytometry/Cell sorting to suggest a potential clinical benefit for WNT suppression."Authors Griffiths EA,Golding MC,Srivastava P,Povinelli BJ,James SR,Ford LA,Wetzler M,Wang ES,Nemeth MJ4.Journal of biomedical materials research. Part AUndifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study.2014"12-0459 was used in Flow cytometry/Cell sorting to assess the ossification ability of adipose-derived stem cells."Authors Carvalho PP,Leonor IB,Smith BJ,Dias IR,Reis RL,Gimble JM,Gomes ME5.Arthritis and rheumatology (Hoboken, N.J.)A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.2014"12-0459 was used in Flow cytometry/Cell sorting to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients."Authors Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

技术参数

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