| Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: The HIT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The HIT2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 40 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.PloS oneHigher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers."14-0389 was used in Flow cytometry/Cell sorting to investigate the frequency, phenotype and activity of T and NK cells in HIV controllers, showing higher frequencies of NK and CD4+ T-Cells and potent cytotoxic response."Authors Taborda NA,González SM,Alvarez CM,Correa LA,Montoya CJ,Rugeles MT 2.Proceedings of the National Academy of Sciences of the United States of AmericaDevelopment of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus."14-0389 was used in Flow cytometry/Cell sorting to study T-cell development in the thymus at a clonal level, with an aim to improve immune reconstitution following hematopoietic stem cell transplant."Authors Brugman MH,Wiekmeijer AS,van Eggermond M,Wolvers-Tettero I,Langerak AW,de Haas EF,Bystrykh LV,van Rood JJ,de Haan G,Fibbe WE,Staal FJ3.Rheumatology (Oxford, England)Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis."14-0389 was used in Flow cytometry/Cell sorting to study the frequencies of different B cell subtypes during progression of ANCA-associated vasculitis."Authors Todd SK,Pepper RJ,Draibe J,Tanna A,Pusey CD,Mauri C,Salama AD4.PloS oneIn vivo expansion of co-transplanted T cells impacts on tumor re-initiating activity of human acute myeloid leukemia in NSG mice."14-0389 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the limitations of xenotransplantation assays of cells from leukaemia patients into immune compromised mice."Authors von Bonin M,Wermke M,Cosgun KN,Thiede C,Bornhauser M,Wagemaker G,Waskow C5.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsB cell repopulation after alemtuzumab induction-transient increase in transitional B cells and long-term dominance of naïve B cells."14-0389 was used in Flow cytometry/Cell sorting to investigate the characteristics of B cells that repopulate following Alemtuzumab induction therapy."Authors Heidt S,Hester J,Shankar S,Friend PJ,Wood KJ |
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