CD140a (PDGFRA) Monoclonal Antibody (APA5), APC

货号：17-1401-81

规格：50 µg

价格：1594

产品类型：流式抗体

品牌：eBioscience

抗原：CD140a (PDGFRA)

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：APA5

荧光染料：APC

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	1 µg/test(Flow）

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD140a (PDGFRA) Antibody (17-1401-81) in FlowStaining of NIH-3T3 cells with 0.5 µg of Rat IgG2a K Isotype Control APC(Product # 17-4321-81) (blue histogram) or 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a) APC(purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Nature communicationsActivin-dependent signaling in fibro/adipogenic progenitors causes fibrodysplasia ossificans progressiva."17-1401 was used in Flow cytometry/Cell sorting to show that fibro/adipogenic progenitors are major cells-of-origin of heterotopic ossification, in an accurate genetic mouse model of fibrodysplasia ossificans progressiva."Authors Lees-Shepard JB,Yamamoto M,Biswas AA,Stoessel SJ,Nicholas SE,Cogswell CA,Devarakonda PM,Schneider MJ,Cummins SM,Legendre NP,Yamamoto S,Kaartinen V,Hunter JW,Goldhamer DJ2.EMBO molecular medicineAttenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung disease."17-1401 was used in Blocking experiments to discover attenuated PDGF signalling as a driver of neonatal chronic lung disease and provision of PDGF-A as a protective strategy."Authors Oak P,Pritzke T,Thiel I,Koschlig M,Mous DS,Windhorst A,Jain N,Eickelberg O,Foerster K,Schulze A,Goepel W,Reicherzer T,Ehrhardt H,Rottier RJ,Ahnert P,Gortner L,Desai TJ,Hilgendorff A3.Stem cell reportsCells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors."17140181 was used in flow cytometry and immunocytochemistry to show that cultured mouse embryonic stem cells express platelet-derived growth factor receptor alpha heterogeneously"Authors Lo Nigro A,de Jaime-Soguero A,Khoueiry R,Cho DS,Ferlazzo GM,Perini I,Abon Escalona V,Aranguren XL,Chuva de Sousa Lopes SM,Koh KP,Conaldi PG,Hu WS,Zwijsen A,Lluis F,Verfaillie CM4.Journal of immunology (Baltimore, Md. : 1950)Ontogeny of stromal organizer cells during lymph node development."17-1401 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells."Authors Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH5.Proceedings of the National Academy of Sciences of the United States of AmericaOlig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.Authors Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ

技术参数

产品应用 Flow；IF; IHC (F)

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