Rat IgG2b kappa Isotype Control, APC

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Rat IgG2b kappa Isotype Control, APC

货号:17-4031-81,17-4031-82

规格:50 µg,100 µg

价格:1387,2350

产品类型:大鼠同型对照

品牌:eBioscience

物种:大鼠

宿主:大鼠

抗体亚型:IgG2b, kappa

克隆号:eB149/10H5

荧光染料:APC

抗体类型:单抗同型对照:IgG2b, kappa
免疫原性:用法:
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: APC Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献:
1.Molecular cancerHOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells."17-4031 was used in Flow cytometry/Cell sorting to to study the role of HOXC8 regulation in breast cancer stem cells."Authors Shah M,Cardenas R,Wang B,Persson J,Mongan NP,Grabowska A,Allegrucci C2.Molecular therapy oncolyticsIntratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer."17-4031 was used in Flow cytometry/Cell sorting to identify microenvironmental factors, which may be decisive for failure or success of oncolytic VACV therapy."Authors Kober C,Weibel S,Rohn S,Kirscher L,Szalay AA3.The Journal of experimental medicineTREM-2 promotes macrophage survival and lung disease after respiratory viral infection."17-4031 was used in Flow cytometry/Cell sorting to define the link between viral infection and type 2 immune response in chronic respiratory disease, showing that expression of TREM2 in macrophages is required, particularly soluble TREM2, which prevents apoptosis of macrophages and leads to chronic inflammatory disease."Authors Wu K,Byers DE,Jin X,Agapov E,Alexander-Brett J,Patel AC,Cella M,Gilfilan S,Colonna M,Kober DL,Brett TJ,Holtzman MJ4.Stem cells and developmentEnrichment of Human Stem-Like Prostate Cells with s-SHIP Promoter Activity Uncovers a Role in Stemness for the Long Noncoding RNA H19."17-4031 was used in Flow cytometry/Cell sorting to provide the first evidence that s-SHIP-GFP promoter reporter offers a unique marker for the enrichment of human stem-like cell populations."Authors Bauderlique-Le Roy H,Vennin C,Brocqueville G,Spruyt N,Adriaenssens E,Bourette RP5.BMC immunologyCd1d regulates B cell development but not B cell accumulation and IL10 production in mice with pathologic CD5(+) B cell expansion."17-4031 was used in Flow cytometry/Cell sorting to investigate the effect of CD1d expression on CD5+ B cell accumulation, IL10 competence and antibody production in mice with pathologic CD5+ B cell expansion."Authors Palmer VL,Nganga VK,Rothermund ME,Perry GA,Swanson PC
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