CD34 Monoclonal Antibody (RAM34), FITC, eBioscience™

货号：11-0341-81,11-0341-82,11-0341-85

规格：50 µg,100 µg,500 µg

价格：964,1488,2979

产品类型：流式抗体

品牌：eBioscience

抗原：CD34

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：RAM34

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, FITC, eBioscience™
浓度：		用法：	1 µg/test(Flow）

产品详细信息Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressedon endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

数据

CD34 Antibody (11-0341-81) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Mouse HematopoieticLineage Biotin Panel (Product # 88-7774-75) followed by Streptavidin PE(Product # 12-4317-87) and 0.5 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42)(left) or 0.5 µg of Anti-Mouse CD34 FITC (right). Total viable cells were used for analysis.

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参考文献：

1.Scientific reportsErythro-myeloid progenitors can differentiate from endothelial cells and modulate embryonic vascular remodeling."11034182 was used in immunocytochemistry to show erythro-myeloid progenitors directly differentiate into endothelial cells"AuthorsKasaai B,Caolo V,Peacock HM,Lehoux S,Gomez-Perdiguero E,Luttun A,Jones EA 2.PloS oneAlkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity."11-0341 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics."AuthorsJonas BA,Johnson C,Gratzinger D,Majeti R 3.PloS oneCharacterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification."11034182 was used in flow cytometry to describe the properties of cells derived from tissue containing pre-and mature ectopic bone using three heterotopic ossification models and patients samples"AuthorsAgarwal S,Drake J,Qureshi AT,Loder S,Li S,Shigemori K,Peterson J,Cholok D,Forsberg JA,Mishina Y,Davis TA,Levi B4.OncotargetTumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism."11034182 was used in flow cytometry to find the specific gene signature related to iron metabolism consisting of genes regulating iron uptake, mitochondrial FeS cluster biogenesis and hypoxic response"AuthorsRychtarcikova Z,Lettlova S,Tomkova V,Korenkova V,Langerova L,Simonova E,Zjablovskaja P,Alberich-Jorda M,Neuzil J,Truksa J5.Scientific reportsErythro-myeloid progenitors can differentiate from endothelial cells and modulate embryonic vascular remodeling."11034182 was used in immunocytochemistry to show erythro-myeloid progenitors directly differentiate into endothelial cells"AuthorsKasaai B,Caolo V,Peacock HM,Lehoux S,Gomez-Perdiguero E,Luttun A,Jones EA

技术参数

产品应用 Flow；IF；ICC；IHC；IHC (F)

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