CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), FITC, eBioscience™

货号：11-0411-81,11-0411-82,11-0411-85

规格：50 µg,100 µg,500 µg

价格：957,1627,3092

产品类型：流式抗体

品牌：eBioscience

抗原：CD41a

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：eBioMWReg30 (MWReg30)

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG1 kappa Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.125 µg/test(Flow）

产品详细信息Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha Iib). While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin.Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC). Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice. Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells. Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells.Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

数据

CD41a Antibody (11-0411-82) in FlowStaining of C57BL/6 platelets with Anti-Mouse/Rat CD61 (Integrin beta 3) PE(Product # 12-0611-82) and 0.06 µg of Rat IgG1 K Isotype Control FITC(Product # 11-4301-82) (left)CD41a Antibody (11-0411-82) in FlowStaining of C57BL/6 platelets with Anti-Mouse/Rat CD61 (Integrin beta 3) PE(Product # 12-0611-82) and 0.06 µg of Rat IgG1 K Isotype Control FITC (Product # 11-4301-82)(left) or 0.06 µg of Anti-Mouse CD41 FITC (right). or 0.06 µg of Anti-Mouse CD41 FITC (right).

推荐产品：

免疫细胞分选CD41a流式抗体细胞因子细胞周期检测细胞凋亡检测细胞增殖活性检测

参考文献：

1.The Journal of clinical investigationLoss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms."11-0411 was used in Flow cytometry/Cell sorting to identify pleckstrin-2 as an effector of the JAK2/STAT5 pathway and a key factor in the pathogenesis of JAK2V617F-induced myeloproliferative neoplasms."AuthorsZhao B,Mei Y,Cao L,Zhang J,Sumagin R,Yang J,Gao J,Schipma MJ,Wang Y,Thorsheim C,Zhao L,Stalker T,Stein B,Wen QJ,Crispino JD,Abrams CS,Ji P 2.Scientific reportsTargeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model. 3.EBioMedicineSmall Molecule Inhibitor of CBFβ-RUNX Binding for RUNX Transcription Factor Driven Cancers.4.Molecular and cellular biologyThe Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.5.Journal of hematology and oncologyH2S protects against fatal myelosuppression by promoting the generation of megakaryocytes/platelets.

技术参数

产品应用 Flow；FN；IF；IHC；WB；IHC (F)；IP

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16978.htm