CD133 (Prominin-1) Monoclonal Antibody (TMP4), APC

货号：17-1338-41,17-1338-42

规格：25 Tests,100 Tests

价格：1411,3361

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：TMP4

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	5uL（0.125µg）/test(Flow）

Description: The TMP4 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites: the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others.The binding of the TMP4 antibody does not block the binding of another anti-human CD133 antibody, EMK08 (cat. 12-1339) indicating that they recognize distinct epitopes.Applications Reported: This TMP4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TMP4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD133 (also named AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. It is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. It has been shown to be expressed in cancers, including some leukemias and brain tumours, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD133 (Prominin-1) Antibody (17-1338-41) in Flow Staining of normal human peripheral blood cells with Anti-Human CD45 PE (Product # 12-9459-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD133 APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. PloS oneEvaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. CytotechnologyImmunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach."17-1338 was used in Flow cytometry/Cell sorting to enrich the stem/progenitor cell population from human umbilical cord blood."AuthorsIndumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M3. PloS oneComparative evaluation of differentiation potential of menstrual blood- versus bone marrow-derived stem cells into hepatocyte-like cells.AuthorsKhanjani S,Khanmohammadi M,Zarnani AH,Akhondi MM,Ahani A,Ghaempanah Z,Naderi MM,Eghtesad S,Kazemnejad S4. Annals of hematologyInhibition of p38 MAPK activity promotes ex vivo expansion of human cord blood hematopoietic stem cells."17-1338 was used in Flow cytometry/Cell sorting to examine the effect of inhibition of p38 mitogen-activated protein kinase on expansion of human umbilical cord blood CD133(+) cells."AuthorsZou J,Zou P,Wang J,Li L,Wang Y,Zhou D,Liu L

技术参数

产品应用 Flow

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