Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647/羊抗豚鼠 IgG (H+L) 高交叉吸附 荧光二抗, Alexa Fluor 647

货号：A-21450

规格：500μl

价格：3761

产品类型：荧光二抗

品牌：Thermo Fisher

物种：其它

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 647

点击查看所有Alexa Fluor荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（IHC）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)

To minimize cross-reactivity, these goat anti-guinea pig IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine, chicken, goat, hamster, human, mouse, rabbit, rat, and sheep sera prior to conjugation. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

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参考文献：

1. Frontiers in neuroanatomyDifferential Inputs to the Perisomatic and Distal-Dendritic Compartments of VIP-Positive Neurons in Layer 2/3 of the Mouse Barrel Cortex."A21450 was used in immunohistochemistry to determine the consequences of vasoactive intestinal polypeptide-positive neuron dendrites in the vertical orientation"AuthorsSohn J,Okamoto S,Kataoka N,Kaneko T,Nakamura K,Hioki H2. Journal of neuroscience researchReceptors Mediate Tonic Inhibition in the Spinal Cord Dorsal Horn and Contribute to the Resolution Of Hyperalgesia."A21450 was used in immunohistochemistry to ask if alpha-5GABA receptors create tonic conductance in the spinal cord dorsal horn to constrain nociception"AuthorsPerez-Sanchez J,Lorenzo LE,Lecker I,Zurek AA,Labrakakis C,Bridgwater EM,Orser BA,De Koninck Y,Bonin RP3. PloS oneA Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System."A21450 was used in immunohistochemistry to generate a single adeno-associated virus vector Tet-Off platform, adeno-associated virus-SynTetOff, to improve the gene-transduction efficiency, specifically in neurons"AuthorsSohn J,Takahashi M,Okamoto S,Ishida Y,Furuta T,Hioki H4. Cell systemsA Single-Cell Transcriptome Atlas of the Human Pancreas."A21450 was used in immunohistochemistry to report the transcriptomes of thousands of single pancreatic cells"AuthorsMuraro MJ,Dharmadhikari G,Grün D,Groen N,Dielen T,Jansen E,van Gurp L,Engelse MA,Carlotti F,de Koning EJ,van Oudenaarden A5. GliaFunctional anisotropic panglial networks in the lateral superior olive."A21450 was used in immunohistochemistry to test if astrocyte networks display properties that reflect precise neuronal arrangement"AuthorsAugustin V,Bold C,Wadle SL,Langer J,Jabs R,Philippot C,Weingarten DJ,Rose CR,Steinhäuser C,Stephan J

技术参数

产品应用 ICC;IF;IHC

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