GST Tag Monoclonal Antibody (8-326)/GST标签抗体

货号：MA4-004,MA4-004-1MG

规格：100 ug,1mg

价格：2753,13782

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：其它

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant purified full-length GST	用法：	2 µg（IP）；1:1000(WB)

Background/Target InformationGST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GST Tag Antibody (MA4-004) in WBWestern blot analysis of GST-Abl SH2 domain (Lane 1, 35 kDa) (Product # 87702) and GST-Grb2 (Lane 2, 38 kDa) SH2 domain (Product # 87700) was performed by loading 2 µg of each purified fusion protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing GST clone 8-826 (Product # MA4-004) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:25000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce SuperSignal West Dura (Product # 34075).

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参考文献：

1.CellC9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles."MA4-004 was used in western blot to report the interactome of all dipeptide repeat proteins"AuthorsLee KH,Zhang P,Kim HJ,Mitrea DM,Sarkar M,Freibaum BD,Cika J,Coughlin M,Messing J,Molliex A,Maxwell BA,Kim NC,Temirov J,Moore J,Kolaitis RM,Shaw TI,Bai B,Peng J,Kriwacki RW,Taylor JP2.BMC immunologyCryptic protein-protein interaction motifs in the cytoplasmic domain of MHCI proteins."MA4-004 was used in western blot to discuss factors involved in major histocompatibility complex class I cytoplasmic signaling"AuthorsFrietze KK,Pappy AL,Melson JW,O'Driscoll EE,Tyler CM,Perlman DH,Boulanger LM3.PloS onePhosphorylation state-dependent interactions of hepadnavirus core protein with host factors."MA4-004 was used in immunoprecipitation to study interactions between host factors and hepadnavirus core protein"AuthorsLudgate L,Adams C,Hu J4.Molecular biology of the cellNovel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly."MA4-004 was used in ELISA to study the functional significance of the interaction of ESCRT III with LIP5 and VPS4"AuthorsShim S,Merrill SA,Hanson PI5.NatureTheileria parasites secrete a prolyl isomerase to maintain host leukocyte transformation."MA4-004 was used in western blot to identify a homologue of the peptidyl-prolyl isomerase PIN1 in T. annulata and investigate how it affects oncogenic signaling pathways."AuthorsMarsolier J,Perichon M,DeBarry JD,Villoutreix BO,Chluba J,Lopez T,Garrido C,Zhou XZ,Lu KP,Fritsch L,Ait-Si-Ali S,Mhadhbi M,Medjkane S,Weitzman JB

技术参数

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