Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

货号：17-4724-41,17-4724-42

规格：25 Tests,100 Tests

价格：957,1599

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：APC

类型:	同型对照	同型对照：
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test（Flow）； Assay-Dependent(Ctrl)

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: The APC conjugated mouse IgG2a isotype control is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 µg/million cells (100 µL blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at test size: 5 µL (1 µg)/test per million cells (100 µL blood).Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

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文献：

1. Journal of immunology (Baltimore, Md. : 1950)Human NK Cells Lyse Th2-Polarizing Dendritic Cells via NKp30 and DNAM-1."17-4724 was used in Flow cytometry/Cell sorting to study the impact of natural killer-dendritic cell interactions in Th1 immune responses and polarising Th2 immunity."AuthorsWalwyn-Brown K,Guldevall K,Saeed M,Pende D,Önfelt B,MacDonald AS,Davis DMYear2018 2. Nature communicationsPDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma."17-4724 was used in Flow cytometry/Cell sorting to elucidate an endothelial plasticity-mediated mechanism that controls anti-angiogenic therapy resistance."AuthorsLiu T,Ma W,Xu H,Huang M,Zhang D,He Z,Zhang L,Brem S,O'Rourke DM,Gong Y,Mou Y,Zhang Z,Fan YYear2018 3. Journal of neuroinflammationPerturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury."17-4724 was used in Flow cytometry/Cell sorting to unveil a novel role for LAR and PTPσ in regulating neuroinflammation in traumatic spinal cord injury."AuthorsDyck S,Kataria H,Alizadeh A,Santhosh KT,Lang B,Silver J,Karimi-Abdolrezaee SYear2018Dilution1:20 4. PloS oneEstradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4."17-4724 was used in Flow cytometry/Cell sorting to demonstrate that Estradiol alters the immune-responsiveness of HECECs to TLR2 and TLR4 ligands by TLR subtype and duration of exposure."AuthorsS Lashkari B,Anumba DOYear2017 5. Journal of virologyThe respiratory syncytial virus fusion protein and neutrophils mediate the airway mucin response to pathogenic respiratory syncytial virus infection."17-4724 was used in Flow cytometry/Cell sorting to elucidate the mechanisms that mediate the airway mucin response to pathogenic respiratory syncytial virus infection."AuthorsStokes KL,Currier MG,Sakamoto K,Lee S,Collins PL,Plemper RK,Moore MLYear2013 6. The Journal of infectious diseasesB and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation."17-4724 was used in Flow cytometry/Cell sorting to characterise B and T lymphocyte attenuator expression and function during HIV-1 infection."AuthorsZhang Z,Xu X,Lu J,Zhang S,Gu L,Fu J,Jin L,Li H,Zhao M,Zhang J,Wu H,Su L,Fu YX,Wang FSYear2011 7. Molecular visionA comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model."17-4724 was used in Flow cytometry/Cell sorting to define the immunologic reaction toward corneal allografts."AuthorsMaenz M,Morcos M,Ritter T

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