TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), PE, eBioscience™

货号：12-8883-82

规格：100 ug

价格：2448

产品类型：流式抗体

品牌：eBioscience

抗原：TRA-1-81

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-81

荧光染料：PE

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Beta-tubulin from sea urchin (S. purpuratus) sperm.	用法：	2.0 µg/mL(ICC);10 µg/mL(IF);1 µg/mL(WB)

Product Specific InformationDescription: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Generation of hepatoblasts from human embryonic stem cells (hESCs). (A) Protocol to differentiate hESCs into progenitors. (B) Images showing the sequential morphological changes that occur to give a polygonal shape after 10 days of culture in appropriate conditions. (C) Immunocytochemistry showing the expression of pluripotency markers (OCT4, NANOG, TRA-1-60, SSEA4) at day 0 (Panel i) followed by the expression of definitive endoderm markers (GATA4, CXCR4, HNF3beta, SOX17) at day 5 (Panel ii) and the expression of hepatic endodermal cells markers (HNF3beta, HNF4alpha, CK19, GATA4, SOX9) at day 8 (Panel iii). At day 10, cells are positive for HNF3beta, AFP, HNF6, HNF4alpha, CK19, and GATA4 and negative for CK7 (Panel iv). (D) Flow cytometry analysis of pluripotency marker TRA-1-81 (Panel i), definitive endoderm marker CXCR4 (Panel ii) at day 5, and hepatic endoderm/progenitor marker EpCAM expression (Panel iii and iv) respectively. Scale bars = 100 mum. TRA-1-81 (Podocalyxin) Antibody (12-8883-82) in FlowStaining of 2102Ep human embryonal carcinoma cell line with 0.5 µg of Mouse IgM Isotype Control PE (blue histogram) or 0.5 µg of Anti-Human TRA-1-81 PE (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Brain researchComparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes."12-8883 was used in Immunocytochemistry to examine the comparative cytotoxicity of 80 compounds in induced pluripotent stem cells(iPSC), isogenic iPSC-derived neural stem cells, neurons and astrocytes."AuthorsPei Y,Peng J,Behl M,Sipes NS,Shockley KR,Rao MS,Tice RR,Zeng X2.Stem cells and developmentSimplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.3.Stem cell reviewsComparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).

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