Nanog Monoclonal Antibody (eBioMLC-51), eBioscience™

货号：14-5761-80

规格：25 µg

价格：2266

产品类型：流式抗体

品牌：eBioscience

抗原：Nanog

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBioMLC-51

荧光染料：Purified

类型:	单抗	同型对照：	IgG2a, kappa
浓度:	0.5 mg/mL	用法：	2.0 µg/mL(ICC);2.0 µg/mL(IF);2.0 µg/mL(WB)

Product Specific InformationDescription: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.Applications Reported: This eBioMLC-51 antibody has been reported for use in western blotting, and immunocytochemistry.Applications Tested: This eBioMLC-51 antibody has been tested by immunoblotting the F9 cell line or by immunocytochemistry of fixed and permeabilized F9 cells. This purified antibody can be used at less than or equal to 2 µg/mL, however should be titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationNANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Nanog Antibody (14-5761-80) in WBLysates from the F9 cell line (left), the NIH/3T3 cell line (middle), and BALB/c spleen (right) were probed with 1 µg/mL of Anti-Mouse Nanog Purified and revealed with Anti-Rat IgG HRP.

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参考文献：

1.Nature cell biologyPluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity.AuthorsFinley LWS,Vardhana SA,Carey BW,Alonso-Curbelo D,Koche R,Chen Y,Wen D,King B,Radler MR,Rafii S,Lowe SW,Allis CD,Thompson CB2.Development (Cambridge, England)A membrane-associated β-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells."14-5761 was used in Western Blotting to investigate the role of a β-catenin/Oct4 complex on pluripotency."AuthorsFaunes F,Hayward P,Descalzo SM,Chatterjee SS,Balayo T,Trott J,Christoforou A,Ferrer-Vaquer A,Hadjantonakis AK,Dasgupta R,Arias AM3.Science advancesAn Mll4/COMPASS-Lsd1 epigenetic axis governs enhancer function and pluripotency transition in embryonic stem cells."14-5761 was used in Immunocytochemistry to define the epigenetic activity of Mll4 and propose Lsd1 opposing regulatory axis which regulates exit of ground-state pluripotency in murine embryonic stem cells."AuthorsCao K,Collings CK,Morgan MA,Marshall SA,Rendleman EJ,Ozark PA,Smith ER,Shilatifard A4.eLifeA lncRNA fine tunes the dynamics of a cell state transition involvingLin28,let-7andde novoDNA methylation."14-5761 was used in Immunocytochemistry to determine the mechanism by which a novel rodent-specific lncRNA delays embryonic stem cells pluripotent execution."AuthorsLi MA,Amaral PP,Cheung P,Bergmann JH,Kinoshita M,Kalkan T,Ralser M,Robson S,von Meyenn F,Paramor M,Yang F,Chen C,Nichols J,Spector DL,Kouzarides T,He L,Smith A5.Nature communicationsA post-transcriptional program coordinated by CSDE1 prevents intrinsic neural differentiation of human embryonic stem cells."14-5761 was used in Immunocytochemistry to identify CSDE1 as a central post-transcriptional regulator of human embryonic stem cells identity and neurogenesis."AuthorsJu Lee H,Bartsch D,Xiao C,Guerrero S,Ahuja G,Schindler C,Moresco JJ,Yates JR,Gebauer F,Bazzi H,Dieterich C,Kurian L,Vilchez D

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