SOX2 Monoclonal Antibody (Btjce), Alexa Fluor 488, eBioscience™

货号：53-9811-82,53-9811-80

规格：100 µg,25 µg

价格：3794,1734

产品类型：流式抗体

品牌：eBioscience

抗原：SOX2

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：Btjce

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.25 µg/mL（Flow）;5 µg/mL(ICC);5 µg/mL(IF)

Product Specific InformationDescription: The Btjce monoclonal antibody reacts with the transcription factor Sox2, a member of the SOX (sex determining region Y -related HMG (High Mobility Group) Box) family of proteins. Sox family members play a role in early organ development, and in particular, Sox2 is essential for regulating genes that control normal mammalian embryogenesis. Sox2 and family member Sox3 are expressed as early as the preimplantation and epiblast stages respectively. Later expression is restricted to the neuroepithelium. Sox2 has been shown to be necessary for maintaining self-renewal and pluripotency of mouse and human embryonic stem (ES) cells (ESC). Oct4 (POU5F1), Klf4, c-myc, and Sox2 were the original four factors used to reprogram differentiated mouse and human cells to induced pluripotent stem cells (iPSC).Expression of Sox2 is tightly regulated and recent studies have demonstrated that small changes in the levels of Sox2 in ES cells can trigger differentiation into multiple cell types. Sox2 expression is not limited to ES cells, it is also essential for early neurogenesis where its expression becomes restricted to the neural plate, and later to neural stem cells where it functions to suppress neural differentiation. Sox2 in combination with other stem cell markers can be used to characterize stem cell populations. Ectopic expression of Sox2 has been associated with multiple cancer types including colorectal and breast.Applications Reported: This Btjce antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This Btjce antibody has been tested by immunocytochemistry on fixed and permeabilized Ntera cells at less than or equal to 10 µg/mL. In flow cytometry it can be used at 0.25 µg/mL using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationSOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SOX2 Antibody (53-9811-82) in ICCImmunocytochemistry on fixed and permeabilized N-tera cells using 10 µg/mL Rat IgG2a Isotype Control (left) or Anti-Human/Mouse Sox2 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

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参考文献：

1.Nature communicationsBCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.2.eLifeMicropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning.3.Development (Cambridge, England)Selection and dynamics of embryonic stem cell integration into early mouse embryos.4.eLifeDynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.5.Development (Cambridge, England)Selection and dynamics of embryonic stem cell integration into early mouse embryos.

技术参数

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