CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), FITC, eBioscience™

货号：11-9886-42

规格：100 Tests

价格：2055

产品类型：流式抗体

品牌：eBioscience

抗原：CD235a

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：10F7MN

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

Product Specific InformationDescription: The monoclonal antibody 10F7MN recognizes human glycophorin A (also known as CD235a). The antibody can see both the M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease.Applications Reported: This 10F7MN antibody has been reported for use in flow cytometric analysis.Applications Tested: This 10F7MN antibody has been pre-titrated and tested by flow cytometric analysis of normal human blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (11-9886-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD235 eFluor® 450 and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human Glycophorin A (MN) FITC (right). Red blood cell gate was used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.BloodErythrocyte sialoglycoproteins engage Siglec-9 on neutrophils to suppress activation.AuthorsLizcano A,Secundino I,Döhrmann S,Corriden R,Rohena C,Diaz S,Ghosh P,Deng L,Nizet V,Varki A2.PloS onePlatelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients."11-9886 was used in Flow cytometry/Cell sorting to investigate whether sites exist on the surfaces of circulating blood cells for the protection of infectious HIV-1 particles."AuthorsBeck Z,Jagodzinski LL,Eller MA,Thelian D,Matyas GR,Kunz AN,Alving CR

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-18097.htm