| Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick.The enzyme acts in a 3′→5′ direction producing stretches of single-stranded (ss) DNA.Exo III is not active on 3′ overhangs of 4 or more bases in length, ssDNA, or on thioester-linked nucleotides. The enzyme also has RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities.Exo III can be heat inactivated by incubation at 65°C for 15 minutes. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix. These include reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios.Exo III is available in 25,000-Unit size at a concentration of 200 Units/µL. 10X Reaction Buffer is provided with the enzyme.核酸外切酶III从切口、平末端或3’凹末端以3’→5’方向消化双链DNA，在相反链上产生ssDNA片段。在65℃温育15分钟，可以对外来III进行加热灭活. |
| 优点： |
| ▪ Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends▪ Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs |
| 组成成分： |
| ▪ Exonuclease III(200U/μL)：储存在-20℃▪ 10X Exonuclease III Reaction Buffer：储存在-20℃ |
| 参考文献： |
| 1. Weiss, B. (1976) J. Biol. Chem. 251, 1896. 2. Rogers, S.G. and Weiss, B. (1980) Meth. Enzymology 65, 201. 3. Sambrook, J. et al., (1989) in: Molecular Cloning: A Laboratory Manual (2nd ed.), Cold Spring Harbor Laboratory Press, New York. 4. Richardson, C.C. et al., (1964) J. Biol. Chem. 239, 251. 5. Guo, L.H. and Wu, R. (1982) Nucl. Acids Res. 10, 2065. |
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