Pierce High Capacity Endotoxin Removal Spin Columns, 1 mL 高容量内毒素去除离心柱，1 mL
货号：88276,88277
规格：5 columns,25 columns
价格：2460,8219
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour; with this spin cup format, protein recovery is ≥85%.The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.Requires:Endotoxin-free waterApplications:Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injectionThe presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery.† One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution. |
| 特点： |
| - High capacity—bind up to 2,000,000 EU/mL† to eliminate >99% of endotoxins- Selective—recover ≥85% of your protein sample- High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL- Fast—our new spin cup format enables endotoxin depletion within 1 hour- Clean—single-use spin columns avoid cross contamination of samples- Optimized—spin cups are optimized for different sample volumes |
| 数据： |
1. Centrifuge at 500Xg for 1 minute to remove the storage buffer. 2. Regenerate with either 0.2N NaOH (overnight) or 0.2N NaOH in 95% ethanol for 1-2 hours. 3. Wash with 2M NaCl followed by ET-free water. 4. Equilibrate with ET-free buffer, pH 6-8. Repeat three times. 5. Add sample and incubate at 4-22°C with gentle end-over-end mixing for 1 hour. 6. Centrifuge at 500 X g for 1 minute to collect the sample. Chemical structure |
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