| The Rat Vascular Endothelial cell Growth Factor A-Cell Lysates (Rt VEGF-A) ELISA quantitates Rt VEGF-A in rat cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Rt VEGF-A.大鼠血管内皮细胞生长因子A细胞裂解物（大鼠VEGF-A）ELISA定量了大鼠细胞或组织裂解物中的大鼠VEGF-A。 该检测试剂盒将专门识别天然和重组大鼠VEGF-A。Principle of the method 方法原理The Rat VEGF-A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.大鼠VEGF-A固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.VEGF（血管内皮生长因子）是一种45 kDa的同型二聚体，二硫键连接的糖蛋白，参与血管生成，可促进肿瘤进展和转移。 VEGF对血管内皮有多种作用，包括促进内皮细胞活力，促有丝分裂，趋化性和血管通透性的能力。 VEGF家族目前包括VEGF-A，VEGF-B，VEGF-C，VEGF-D，VEGF-E和PIGF。 VEGF及其受体系统已被证明是血管新生细胞信号传导中的基本调节剂。大多数肿瘤对血管生成有绝对的要求，而VEGF被描述为与此过程相关的最有效的血管生成细胞因子。迄今为止，已经描述了5种不同的VEGF同工型。这些同工型是从单个VEGF基因进行选择性剪接的结果。各种同工型与两个酪氨酸激酶受体flt-1（VEGFR-1）和flk-1 / KDR（VEGFR-2）结合，发现几乎只在内皮细胞上表达。 VEGF及其高亲和力结合受体酪氨酸激酶FLK1和FLT1认为对胚胎脉管系统的发育很重要。研究表明，FLT1的另一种剪接形式可产生称为sFLT1的可溶性蛋白，该蛋白以高亲和力结合血管内皮生长因子，在血管生成中起抑制作用。 VEGF水平升高与POEMS综合征（多发性神经病，器官肿大，内分泌病，单克隆丙种球蛋白病，皮肤变化）有关，也被称为Crow-Fukase综合征，它会影响体内的多个器官。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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