The InstantOne™ ELISA is specifically engineered for accurate measurement of total human and mouse JNK 1/2 in cell lysates. Detection of rat JNK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.InstantOne™ELISA是专门设计用于精确测量细胞裂解物中人和小鼠总JNK 1/2的量。 预期会检测到大鼠JNK 1/2。 InstantOne™ELISA试剂盒可在大约一小时内快速分析样品。 将传统的夹心ELISA中使用的所有试剂均以溶液形式添加到板上，然后进行洗涤步骤，并使用TMB比色底物进行检测。Target information 靶标信息C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.C-Jun N末端激酶（JNK）也称为丝裂原激活的蛋白激酶（MAPK8）。 它属于应激激活蛋白激酶的MAPK超家族。 MAPK是丝氨酸-苏氨酸蛋白激酶，可响应多种细胞外刺激而被激活，并介导从细胞表面到细胞核的信号转导。 JNK参与多种细胞过程，例如增殖，分化，转录调控和发育。 JNK途径被应激和炎性信号激活。 由于差异的mRNA剪接，JNK被表达为十种不同的亚型。 主要形式为JNK1和JNK2。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用，不用于诊断过程，未经明确授权不得转售。
数据：
JNK1/JNK2 (Total) Multispecies InstantOne™ ELISA KitRepresentative data for JNK1/JNK2 (Total) Multispecies InstantOne™ ELISA. HEK293 cells untreated (-) or treated with 2 µg/mL anisomycin for 30 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the bands at 46 and 54 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.JNK1 / JNK2（Total）多物种InstantOne™ELISA的代表性数据。 未处理（-）或用2 µg / mL Anisomycin处理30分钟（+）的HEK293细胞。 通过免疫印迹（左）和InstantOne™ELISA（右）分析细胞。 通过免疫印迹检测到的46和54 kDa的条带强度与InstantOne™ELISA吸收值相关。
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