BHQ-2 DMT Amidite
货号：BNS-5052-50,BNS-5052-100,BNS-5052-250
规格：50 mg,100 mg,250 mg
价格：2462.25,4572.75,11256
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ-2 amidite is used for the 5' labeling of fluorogenic probes or to place the quencher internally. This amidite contains a DMT protecting group, which is removed under traditional conditions. The BHQ-2 quencher was designed to have maximal absorption in the 560 to 670 nm range, which provides excellent quenching of fluorophores emitting in this range. |
| 特性： |
| - Chemical Name:4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-2-oxy ethyl (4,4' dimethoxy trityl))-N-ethyl-
2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite- Formula:C54H61N8O9P- Molecular Weight:997.08- Appearance:purple crystal- Absorption Maximum (Lambda Max):579- Extinction Coefficient at Lambda max:38000- Extinction Coefficient at 260 nm:8000 |
| 产品用途： |
Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.Dilution:100 µmol/mLDeprotection conditions:Deprotection conditions are dependent upon the type of amidites used for the synthesis of the oligo. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60 °C. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting the dye labeled oligonucleotide in t-butylamine/H2O (1:3) for 12-16 hours at 60 °C.If all the solution is not used during the synthesis, it can be stored under argon and its functionality maintained up to 48 hours.
BHQ-2 amidite is susceptible to oxidation when left out in the open or undessicated.
For detailed information on BHQ fragmentation by MALDI-TOF MS, refer to your BHQ Licensee's Manual Appendix V.Image of cleaved and deprotected structure:The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 556.46 |
| 数据： |
| |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-20846.htm
QQ:1749072012