Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin/兔抗小鼠IgG Fc二抗，Biotin

货号：31813

规格：1.5 mL

价格：4086

产品类型：荧光二抗

品牌：Thermo Fisher

物种：小鼠

宿主：兔

抗体亚型：IgG

荧光染料：Biotin

类型:	二抗	同型对照：
浓度：	1.5 mg/mL	用法：	1:20,000-1:400,000(ELISA)；1:200-1:1,000(Flow)；1:500-1:5,000(ICC)；1:500-1:5,000(IF)；1:500-1:5,000(IHC)；1:500-1:5,000(IP)；1:5,000-1:20,000(WB)

产品详细信息Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.Product # 31813 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.Product # 31813 reacts with the heavy chains of mouse IgG, but not with the light chains common to most mouse immunoglobulins. This antibody does not react against mouse IgM, or against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Reconstitute with 1.5 mL of distilled water.Country of Origin: USA靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Mouse IgG Fc Secondary Antibody (31813) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence using Rabbit anti-Mouse IgG Fc Secondary Antibody, Biotin (Product # 31813) at dilutions 1:5,000 (Fig.1), 1:10,000 (Fig.2) and 1:20,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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