Goat anti-Mouse IgG2a Secondary Antibody, HRP/山羊抗小鼠IgG2a二抗，HRP

货号：A-10685

规格：200 µg

价格：1942

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Mouse IgG2a

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：		用法：	1:500-1:2,000 (ELISA)； 1:500-1:2,000 (WB)； 1:500-1:2,000 (IHC)

产品详细信息Storage and reconstitution: when stored desiccated at or below -20°C, the lyophilized powder is stable for at least one year. Reconstituted using 200 µL PBS, pH 7.2, to yield a 1 mg/mL stock solution. 靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Mouse IgG2a Secondary Antibody (A-10685) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1) and U-2 OS (Lane 2). The blots were probed with Anti-LKB1 Mouse Monoclonal Antibody (Product # AHO1392, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG2a Secondary Antibody, HRP conjugate (Product # A-10685) at dilutions 1:500 (Fig. 1), 1:1,000 (Fig. 2) and 1:2,000 (Fig. 3). A 55 kDa band corresponding to LKB1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. PLoS neglected tropical diseasesSpecies-Specific Immunodetection of an Entamoeba histolytica Cyst Wall Protein.2. Human vaccines and immunotherapeuticsRotavirus capsid VP6 protein acts as an adjuvant in vivo for norovirus virus-like particles in a combination vaccine.3. Human vaccines and immunotherapeuticsComparative immunogenicity in mice of rotavirus VP6 tubular structures and virus-like particles.

技术参数

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