IL-22 Monoclonal Antibody (IL22JOP), Functional Grade

货号：16-7222-82,16-7222-85

规格：100μg,500μg

价格：1328,3389

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：IL22JOP

荧光染料：其它

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade
浓度：	1 mg/mL	用法：	Assay-Dependent(FN)；Assay-DependentNeu)

产品详细信息Description: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. IL-22 is a 20 kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells. Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells. This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo. Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut. Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients. Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis.IL22JOP is published to recognize rhesus IL-22.Applications Reported: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22.Applications Tested: The ND50 of IL22JOP, as determined by the inhibition of IL-10 induction in COLO205 cells by IL-22, is 10-40 ng/mL, in the presence of 0.2 ng/mL recombinant human or mouse IL-22. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IL-22 also known as IL-10-related T-cell derived inducible factor, is an alpha helical cytokine and is considered a member of the IFN-IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and the type I and II interferons. IL-22 is produced mainly by activated T cells and NK cells. In humans, the IL-22 gene is located on the q arm of chromosome 12, and is structurally related to IL10. IL-22 acts by engaging the heterodimeric receptor complex consisting of primary receptor IL-22R1 and accessory receptor IL-10R2. IL-22R1 also binds IL-20 and IL-24; IL-10R2 also binds IL-10, IL-27, IL-28, and IL-29. Binding of IL-22 to its receptor complex induces signal transduction, particularly via the JAK-STAT pathway. In addition to the membrane-bound IL-22R1/IL-10R2 complex, a soluble single-chain IL-22 receptor termed IL-22BP has been found to antagonize IL-22 binding and signaling. IL-22 appears not to directly influence immune cells, and major targets of the cytokine appear to be nonimmune cells, such as cells of the skin, digestive and respiratory system, as well as hepatocytes, and keratinocytes. IL-22 has been described as an effector cytokine of the Th17 lineage. Along with IL-17A and IL-17F, IL-22 regulates genes associated with innate immunity of the skin. IL-17A, IL-17F and IL-22 are all co-expressed by Th17 cells, however, they are differentially regulated. The effects of IL-22 include induction of acute phase reactants and antimicrobial proteins, as well as increasing the mobility of keratinocytes. IL-22 is highly expressed during chronic inflammation, and found to activate intracellular kinases and transcription factors. IL-22 is critical for host defense against infections of extracellular pathogens, and promotes wound-healing responses. IL-22 is upregulated in activated T cells. IL-22 has been reported to mediate IL-23-induced acanthosis and dermal inflammation through activation of STAT3.

数据

IL-22 Antibody (16-7222-82) in FNNeutralization of IL-22 Recombinant Protein activity as measured by IL-10 induction in COLO205 cells.

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参考文献：

1.Frontiers in immunologyATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases."Published figure using IL-22 monoclonal antibody (Product # 16-7222-82) in Flow Cytometry"2.Nature communicationsWASH maintains NKp46+ILC3 cells by promoting AHR expression."16-7222 was used in Flow cytometry/Cell sorting to study how WASH-mediated AHR expression has a critical function in the maintenance of NKp46(+) ILC3s."3.JCI insightProteomics analysis reveals a Th17-prone cell population in presymptomatic graft-versus-host disease."16-7222 was used in Flow cytometry/Cell sorting to discover an ICOS-stimulated, Th17-prone CD4(+)CD146(+)CCR5(+) T cell subset that may serve as an early gastrointestinal graft-versus-host-disease biomarker."AuthorsLi W,Liu L,Gomez A,Zhang J,Ramadan A,Zhang Q,Choi SW,Zhang P,Greenson JK,Liu C,Jiang D,Virts E,Kelich SL,Chu HW,Flynn R,Blazar BR,Hanenberg H,Hanash S,Paczesny S4.JCI insightProteomics analysis reveals a Th17-prone cell population in presymptomatic graft-versus-host disease."16-7222 was used in Flow cytometry/Cell sorting to discover an ICOS-stimulated, Th17-prone CD4(+)CD146(+)CCR5(+) T cell subset that may serve as an early gastrointestinal graft-versus-host-disease biomarker."AuthorsLi W,Liu L,Gomez A,Zhang J,Ramadan A,Zhang Q,Choi SW,Zhang P,Greenson JK,Liu C,Jiang D,Virts E,Kelich SL,Chu HW,Flynn R,Blazar BR,Hanenberg H,Hanash S,Paczesny S5.Nature communicationsAntibiotics in neonatal life increase murine susceptibility to experimental psoriasis."Published figure using IL-22 monoclonal antibody (Product # 16-7222-82) in Flow Cytometry"

技术参数

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