CD62P (P-Selectin) Monoclonal Antibody (AK-4), FITC

货号：11-0628-41,11-0628-42

规格：25 Tests,100 Tests

价格：0,2022

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：AK-4

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (1 μg)/test(Flow)

产品详细信息Description: The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the surface. CD62P initiates the adhesive interaction between endothelial cells and neutrophils and monocytes during the inflammatory reaction and is also involved in the interaction of platelets with monocytes and neutrophils.Applications Reported: The AK-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This AK-4 antibody has been pre-titrated and tested by flow cytometric analysis of resting and thrombin-activated human platelets. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes tothe plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

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参考文献：

1.Oncology reportsActivation of platelet protease-activated receptor-1 induces epithelial-mesenchymal transition and chemotaxis of colon cancer cell line SW620."11-0628 was used in Flow cytometry/Cell sorting to examine the role of protease-activated receptor-1-stimulated platelet activation in the epithelial-mesenchymal transition, migration of colon cancer cells, and to identify the underlying mechanisms."AuthorsJia Y,Zhang S,Miao L,Wang J,Jin Z,Gu B,Duan Z,Zhao Z,Ma S,Zhang W,Li Z

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