| The Human Tumor Necrosis Factor alpha (Hu TNF-α) ELISA quantitates Hu TNF-α in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNF-α.人肿瘤坏死因子α（人TNF-α）ELISA定量检测人血清，血浆，缓冲液或细胞培养基中的人TNF-α。 该检测试剂盒将专门识别天然和重组人NF-α。Principle of the method 方法原理The Human TNF-α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人TNF-α固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.TNFα是一种多功能促炎细胞因子，属于肿瘤坏死因子（TNF）超家族。这种细胞因子主要由巨噬细胞分泌，并与其受体TNFRSF1A / TNFR1和TNFRSF1B / TNFBR结合。 TNFα参与免疫细胞的调节，细胞增殖，分化，凋亡，脂质代谢和凝血。 TNFα以两个，三个或五个非共价连接的单元的多聚体形式存在，但在非还原条件下SDS-PAGE后显示一个17 kDa的条带。小鼠的基因敲除研究还表明了TNFα的神经保护功能，并且已经观察到当注射到荷瘤小鼠中时会引起肿瘤坏死。 TNF-α的其他功能包括其在对细菌，病毒，寄生虫和某些真菌感染的免疫反应中的作用，以及在特定肿瘤坏死中的作用。 TNFα导致某些转化细胞的细胞溶解或细胞停滞，在干扰素方面与干扰素-γ协同作用。该细胞因子与多种疾病有关，包括自身免疫性疾病，胰岛素抵抗和癌症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
QQ:1749072012