TIP60 Polyclonal Antibody

货号：PA5-34548

规格：100 µg

价格：5548

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A 15 amino acid peptide near the carboxy terminus

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	20 µg/mL(ICC) ;20 µg/mL(IF);25 µg/mL(IHC); 1-2 µg/mL(WB)

产品详细信息A suggested positive control is human brain tissue lysate.PA5-34548 can be used with blocking peptide PEP-1591. 靶标信息TIP60 is a transcription factor, originally isolated as an HIV-1 TAT-interactive protein, known to interact with the viral TAT protein that leads to HTATIP polyubiquitination and targets it for degradation. It belongs to the MYST family of histone acetyl transferases (HATs). TIP60 is a catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. A co-regulator of transcription factors, TIP60 either promotes or suppresses tumorigenesis. TIP60 is an enzyme that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

数据：

TIP60 Antibody (PA5-34548) in IF Immunofluorescence analysis of TIP60 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-TIP60 Rabbit Polyclonal Antibody (Product # PA5-34548) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. TIP60 Antibody (PA5-34548) in IHCImmunohistochemical staining of rat brain tissue using a KAT5 polyclonal antibody (Product # PA5-34548) at a 25 µg/mL dilution.

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