CD180 (RP105) Monoclonal Antibody (MHR73-11), Functional Grade, eBioscience™/CD180 (RP105) 单抗 (MHR73-11), 功能抗体, eBioscience™

货号：16-1809-82,16-1809-025,16-1809-050

规格：100 µg,25mg,50mg

价格：2684,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD180

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：MHR73-11

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The MHR73-11 monoclonal antibody reacts with human CD180 (RP105). This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on B, monocytes/macrophages, and dendritic cells. Histological studies show that RP105 is expressed mainly on mature B cells in mantle zones, while germinal center cells are either dull or negative. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. MHR73-11 activates B cells, leading to increases in cell size, expression of the costimulatory molecule CD80, and DNA synthesis. Moreover, ligation of RP105 protects B cells from irradiation- or dexamethasone-induced apoptosis. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death. A significant proportion of circulating B cells in SLE patients is RP105 negative. Loss of RP105 is associated with B cell activation and increased disease activity in SLE patients.Applications Reported: MHR73-11 has been reported for use in flow cytometric analysis. MHR73-11 has also been reported in in vitro functional studies.Applications Tested: The MHR73-11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD180 (RP105, Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. CD180 cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size. CD180 consists of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is part of the cell surface receptor complex, RP105/MD-1. Diseases associated with CD180 dysfunction include Kawasaki Disease in children and may play a role in rheumatic disease pathology.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD180 (RP105) Antibody (16-1809-82) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD180 (RP105) PE.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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参考文献：

功能检测1.Cancer researchAntibody-drug conjugates for the treatment of non-Hodgkin's lymphoma: target and linker-drug selection."14-5931 was used in functional experiments to show the role of MKP5 in restraining p38 MAPK-mediated neutrophil oxidant production, thereby preventing LPS-induced vascular injury in mice."AuthorsPolson AG,Calemine-Fenaux J,Chan P,Chang W,Christensen E,Clark S,de Sauvage FJ,Eaton D,Elkins K,Elliott JM,Frantz G,Fuji RN,Gray A,Harden K,Ingle GS,Kljavin NM,Koeppen H,Nelson C,Prabhu S,Raab H,Ross S,Slaga DS,Stephan JP,Scales SJ,Spencer SD,Vandlen R,Wranik B,Yu SF,Zheng B,Ebens A

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