CD154 (CD40 Ligand) Monoclonal Antibody (24-31), Functional Grade, eBioscience™/CD154 (CD40 Ligand) 单克隆 (24-31), 功能抗体, eBioscience™

货号：16-1548-82,16-1548-38,16-1548-025,16-1548-050

规格：500 µg,5 mg,25 mg,50 mg

价格：2923,24089,72266,120443

产品类型：抗体和染料

品牌：eBioscience

抗原：CD154

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：24-31

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience
浓度：	1 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activationand induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro .Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of activated normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD154 (CD40 Ligand) Antibody (16-1548-82)Oncology letters 2018 -Figure 1. The remnants of LM8 decreased the co-stimulatory molecules, and inhibited IL-12 and IL-6 levels, increased IL-10 levels, and inhibited the ability of DCs to stimulate T-cell proliferation. (A) The DCs were isolated following a 48-h co-culture with the remnants of LM8 cells. The cells were then labeled with antibodies against CD11c, MHC-2, CD40, CD86, CD80 and CCR7 for phenotypic analysis by flow cytometry. The numbers in the histograms indicate the geometric mean fluorescence intensity. (B) Following isolation from the co-culture system, the DCs were cultured for 12 h. Subsequently, the expression levels of IL-12, IL-6 and IL-10 in the supernatant were analyzed by ELISA. (C) CD4 + T cells from DO11.10 OVA 323-339 -specific (TCR-transgenic x C57BL/6) F1 hybrid mice were co-cultured with DCs or mDCs (control) in the presence of OVA peptides. Five days later, the total number of viable CD4 + T (CD4 + 7-AAD - ) cells in each well was measured by flow cytometry. Data represent one of at least three experiments with similar results. *P<0.05 LM8 DC/T vs. Control DC/T. IL, interleukin; DCs, dendritic cells; CD, cluster of differentiation; MHC-2, major histocompatibility complex 2; CCR7, C-C chemokine receptor 7; DC/T, DCs and T cell co-culture; mDCs, mature DCs.

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参考文献：

流式1.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 16-1548-82) in Flow Cytometry"2.The Journal of clinical investigationCD4⁺ and CD8⁺ T cell-dependent antiviral immunity requires STIM1 and STIM2."16-1548 was used in Flow cytometry/Cell sorting to investigate the role of STIM1 and STIM2 in CD4+ and CD8+ T cell-dependent antiviral immunity."AuthorsShaw PJ,Weidinger C,Vaeth M,Luethy K,Kaech SM,Feske S3.BloodComplement-induced regulatory T cells suppress T-cell responses but allow for dendritic-cell maturation."16-1548 was used in Flow cytometry/Cell sorting to show that, despite their IL-10 production, CD46-induced T-regulatory T cells do not suppress the activation/maturation of dendritic cells."AuthorsBarchet W,Price JD,Cella M,Colonna M,MacMillan SK,Cobb JP,Thompson PA,Murphy KM,Atkinson JP,Kemper C功能检测4.Cell reportsChronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells."16-1548 was used in Functional assays to investigate the role of T helper cells in the mechanisms that promote chronic lymphocytic leukemia (CLL) cell survival and proliferation, showing that T helper cells could activate CLL cells in a contact- and CD40L-dependent manner."AuthorsOs A,Bürgler S,Ribes AP,Funderud A,Wang D,Thompson KM,Tjønnfjord GE,Bogen B,Munthe LA中和5.Journal of immunology (Baltimore, Md. : 1950)Polysaccharide-specific memory B cells generated by conjugate vaccines in humans conform to the CD27+IgG+ isotype-switched memory B Cell phenotype and require contact-dependent signals from bystander T cells activated by bacterial proteins to differentiate into plasma cells."16-1548 was used in Blocking experiments to highlight that any protection conferred by PS-specific BMEM at the time of bacterial encounter will depend on the effectiveness with which bacterial proteins are able to activate bystander T cells."AuthorsClarke ET,Williams NA,Findlow J,Borrow R,Heyderman RS,Finn A

技术参数

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