CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Functional Grade, eBioscience™/CD154 (CD40 Ligand) 单克隆 (MR1), 功能抗体, eBioscience™

货号：16-1541-82,16-1541-85,16-1541-38,16-1541-025,16-1541-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1752,3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD154

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：MR1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.Applications Reported: The MR1 antibody has been reported for use in flow cytometric analysis. It has also been reported to inhibit binding of CD154 to CD40 and activation of B cells.Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.仅用于科研。不用于诊断过程。

数据

CD154 (CD40 Ligand) Antibody (16-1541-82) in FlowStaining of 6-hour, PMA/Ionomycin-stimulated T cells from BALB/c spleen with 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (open histogram) or 0.25 µg of Anti-Mouse CD154 Functional Grade Purified (right) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

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参考文献：

流式1.Journal of immunology (Baltimore, Md. : 1950)Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas."16-1541 was used in Flow cytometry/Cell sorting to elucidate the role of CD40 signalling through cognate T-B interaction in the generation of germinal centre and memory B cells."AuthorsBolduc A,Long E,Stapler D,Cascalho M,Tsubata T,Koni PA,Shimoda M2.PloS oneCancer immunotherapy employing an innovative strategy to enhance CD4+ T cell help in the tumor microenvironment."16-1541 was used in Flow cytometry/Cell sorting to investigate the effect of intratumoral injection of PADRE and the adjuvant CpG into HPV16 E7-expressing TC-1 tumours following cisplatin chemotherapy, showing potent antitumor effects and antigen-specific cell-mediated immune response."AuthorsSong L,Yang MC,Knoff J,Wu TC,Hung CF功能检测3.The Journal of investigative dermatologyNatural killer T cells are essential for the development of contact hypersensitivity in BALB/c mice."16-1541 was used in Flow cytometry/Cell sorting to report the pivotal contribution of dendritic and natural killer T cell interactions to hypersensitivity."AuthorsShimizuhira C,Otsuka A,Honda T,Kitoh A,Egawa G,Nakajima S,Nakashima C,Watarai H,Miyachi Y,Kabashima K4.Journal of immunology (Baltimore, Md. : 1950)The Toll-IL-1R member Tir8/SIGIRR negatively regulates adaptive immunity against kidney grafts."16-1541 was used in Functional assays to test the hypothesis that Toll-IL-1R8, a negative regulator of TLR/IL-1R highly expressed in the kidney, modulates immune cell activation underlying kidney rejection."AuthorsNoris M,Cassis P,Azzollini N,Cavinato R,Cugini D,Casiraghi F,Aiello S,Solini S,Cassis L,Mister M,Todeschini M,Abbate M,Benigni A,Trionfini P,Tomasoni S,Mele C,Garlanda C,Polentarutti N,Mantovani A,Remuzzi G中和5.BloodPreventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders."16-1541 was used in Blocking experiments to study the requirements for the restimulation of FVIII-specific memory B cells and their differentiation into anti-FVIII antibody-producing cells."AuthorsHausl C,Ahmad RU,Schwarz HP,Muchitsch EM,Turecek PL,Dorner F,Reipert BM

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