TheQuant-iT RNA Assay Kitmakes RNA quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. The assay is highly selective for RNA over double-stranded DNA, and in the range of 5-100 ng of RNA, the fluorescence signal is linear. Quant-iT™ DNA Assay Kits (Q33120, Q33130) are also available.
数据
Linear Detection Range of Qubit/Quant-iT RNA AssayThe Qubit/Quant-iT RNA Assay has a linear detection range of 5–100 ng in sample volumes of 1–20 µL and is selective for RNA, even in the presence of an equal mass of DNA. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. In the 1:1 mixture, the total mass of nucleic acid at a given point is double what is stated on the x-axis.
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