IFN gamma Monoclonal Antibody (R4-6A2), Functional Grade, eBioscience™/IFN gamma单克隆(R4-6A2), 功能抗体, eBioscience™

货号：16-7312-81,16-7312-85,16-7312-38,16-7312-025,16-7312-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,3315,21041,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IFN gamma

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：R4-6A2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）；Assay-Dependent（功能检测）

产品详细信息Description: The R4-6A2 antibody reacts with mouse interferon-gamma (IFN-gamma ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-gamma is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.Applications Reported: The R4-6A2 antibody has been reported for use in cytokine neutralization.Applications Tested: The Functional Grade Purified R4-6A2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested in bioassay for neutralization of IFN gamma bioactivity.The R4-6A2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.仅用于科研。不用于诊断过程。

数据

IFN gamma Antibody (16-7312-81)Oncoimmunology 2020 -Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81)

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参考文献：

ELISA1.OncoimmunologyCD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b+T cell lymphoma."Published figure using IFN gamma monoclonal antibody (Product # 16-7312-81) in ELISA"2.Journal of immunology (Baltimore, Md. : 1950)The kinetics of myelin antigen uptake by myeloid cells in the central nervous system during experimental autoimmune encephalomyelitis."16-7312 was used in an ELISpot assay to investigate the kinetics of myelin Ag uptake by CNS APCs during experimental autoimmune encephalomyelitis and in the naive CNS."AuthorsSosa RA,Murphey C,Ji N,Cardona AE,Forsthuber TG3. Journal of immunology (Baltimore, Md. : 1950)Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization."16-7312 was used in an ELISpot assay to study the regulation of unique Th cell subset calcium signals and their correlation to biological functions, showing that Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization."AuthorsWeber KS,Hildner K,Murphy KM,Allen PM功能检测4.Antimicrobial agents and chemotherapyImmunotherapy markedly increases the effectiveness of antimicrobial therapy for treatment of Burkholderia pseudomallei infection."16-7312 was used in Functional assays to investigate whether active immunotherapy with IFN-gamma could enhance therapy for B. pseudomallei infection."AuthorsPropst KL,Troyer RM,Kellihan LM,Schweizer HP,Dow SW中和5.Cell death and diseaseSevere influenza A(H1N1)pdm09 infection induces thymic atrophy through activating innate CD8(+)CD44(hi) T cells by upregulating IFN-γ."16-7312 was used in Neutralization experiments to demonstrate that thymic innate CD8(+)CD44(hi) single positive T-cells have critical roles in influenza A(H1N1)pdm09 infection-induced thymic atrophy through secreting IFN-γ."AuthorsLiu B,Zhang X,Deng W,Liu J,Li H,Wen M,Bao L,Qu J,Liu Y,Li F,An Y,Qin C,Cao B,Wang C6.The Journal of experimental medicineRole of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction."16-7312 was used in Neutralization experiments to identify a role for T cells in hypertension and support a role of inflammation in the basis of hypertension."AuthorsGuzik TJ,Hoch NE,Brown KA,McCann LA,Rahman A,Dikalov S,Goronzy J,Weyand C,Harrison DG

技术参数

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