CD8a Monoclonal Antibody (OKT8 (OKT-8)), Functional Grade, eBioscience™/CD8a 单克隆 (OKT8 (OKT-8)),功能抗体, eBioscience™

货号：16-0086-85,16-0086-38,16-0086-025,16-0086-050

规格：500 µg,5 mg,25mg,50 mg

价格：3779,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：OKT8 (OKT-8)

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）;Assay-Dependent（对照）;Assay-Dependent（功能检测）

产品详细信息Description: The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT8 (OKT-8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.仅用于科研。不用于诊断过程。

数据

CD8a Antibody (16-0086-85)Frontiers in pharmacology 2020 -FIGURE 5 Effects of disruption of PD-1 on cytotoxicity, cytokines production, and phenotype of GPC3-CAR T cells in vitro . (A) Cytotoxic activities of UTD and GPC3-CAR T cells with intact or deficient PD-1 against GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) and GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (B,C) The production of IL-2 (B) and IFN-gamma (C) by the UTD and GPC3-CAR T cells with intact or deficient PD-1 cocultured with GPC3-positive (PLC/PRF/5 and SK-HEP-1/GPC3) or GPC3-negative (SK-HEP-1) HCC cells at an effector:target ratio of 1:1. (D) CD4 and CD8 subsets constitution of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD4 and CD8 on CAR T cells were measured by flow cytometry. (E) The expressions of early (CD69), and intermediate or late (CD25) activation markers on cell surface of wild-type and PD-1-deficient GPC3-CAR T cells. The expressions of CD25 and CD69 were determined by flow cytometry. Data shown were mean +- SD from triplicates. Bars, SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 by one way ANOVA with Turkey post hoc test.

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参考文献：

流式1.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."Published figure using CD8a monoclonal antibody (Product # 16-0086-81) in Flow Cytometry"2.PloS oneCCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues."Published figure using CD8a monoclonal antibody (Product # 16-0086-81) in Flow Cytometry"3. Journal of immunology (Baltimore, Md. : 1950)Simian immunodeficiency virus-induced changes in T cell cytokine responses in cynomolgus macaques with latent Mycobacterium tuberculosis infection are associated with timing of reactivation."16-0086 was used in Flow cytometry/Cell sorting to investigate alterations in multifuctional T cell responses during HIV-M. tuberculosis coinfection in an animal model."AuthorsMattila JT,Diedrich CR,Lin PL,Phuah J,Flynn JL功能检测4.BiochemistryMechanistic insight into pertussis toxin and lectin signaling using T cells engineered to express a CD8α/CD3ζ chimeric receptor."16-0086 was used in Functional assays to investigate whether the lectin-like activity of pertussis toxin is responsible for its ability to activate TCR signalling."AuthorsSchneider OD,Millen SH,Weiss AA,Miller WEIF5.The Journal of biological chemistryStructure and Membrane Binding Properties of the Endosomal Tetratricopeptide Repeat (TPR) Domain-containing Sorting Nexins SNX20 and SNX21."Published figure using CD8a monoclonal antibody (Product # 16-0086-81) in Immunofluorescence"中和5.Oncology lettersThe regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10."16-7108 was used in Blocking experiments to investigate the role of exosomes in tumourigenesis."

技术参数

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