CD85j (ILT2) Monoclonal Antibody (HP-F1), Functional Grade, eBioscience™/CD85j (ILT2) 单克隆 (HP-F1), 功能抗体, eBioscience™

货号：16-5129-82,16-5129-38,16-5129-025,16-5129-050

规格：100 µg,5 mg,25 mg,50 mg

价格：2103,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD85j (ILT2)

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：HP-F1

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T andB cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F.The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HP-F1 antibody has been tested by flow cytometric analysis of normal human peripheral cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.仅用于科研。不用于诊断过程。

数据

CD85j (ILT2) Antibody (16-5129-82) in FlowNormal human peripheral blood cells were stained with 0.06 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-85) (blue histogram) or 0.06 µg of CD85j Monoclonal Antibody, Functional Grade (purple histogram) followed by Anti-IgG1 Monoclonal Antibody, Biotin (Product # 13-4015-82) and Streptavidin, PE (Product # 12-4317-87). Cells in the monocyte gate were used for analysis.

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参考文献：

流式1.Journal of immunology (Baltimore, Md. : 1950)Degenerate recognition of MHC class I molecules with Bw4 and Bw6 motifs by a killer cell Ig-like receptor 3DL expressed by macaque NK cells."16-5129 was used in Flow cytometry/Cell sorting to describe the first in-depth characterisation of KIR-MHC interactions in pigtailed macaques to elucidate the role of NK cells in SIV."AuthorsMaloveste SM,Chen D,Gostick E,Vivian JP,Plishka RJ,Iyengar R,Kruthers RL,Buckler-White A,Brooks AG,Rossjohn J,Price DA,Lafont BA2.Proceedings of the National Academy of Sciences of the United States of AmericaSynergistic inhibition of natural killer cells by the nonsignaling molecule CD94."16-5129 was used in Flow cytometry/Cell sorting to elucidate the benefits of having two distinct inhibitory receptor-ligand systems to inhibit natural killer cells."AuthorsCheent KS,Jamil KM,Cassidy S,Liu M,Mbiribindi B,Mulder A,Claas FH,Purbhoo MA,Khakoo SI

技术参数

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