IL-15 Monoclonal Antibody (AIO.3), Functional Grade, eBioscience™ 4篇引用文献/IL-15 单克隆 (AIO.3), 功能抗体, eBioscience™

货号：16-7154-81,16-7154-85,16-7154-38,16-7154-025,16-7154-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：2923,5255,23374,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-15

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：AIO.3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgG2a, lambda
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The AIO.3 antibody reacts with mouse IL-15. This is a neutralizing antibody. Mouse IL-15 is a 14-15 kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the beta and gamma subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NKcell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.Applications Reported: The AIO.3 antibody is reported for use in neutralization of mouse IL-15 bioactivity.Applications Tested: The Functional Grade Purified AIO.3 antibody has been tested by LAL assay to verify low endotoxin levels of < 0.01 ng/ug and in bioassay for neutralization of mouse IL-15 bioactivity.The AIO.3 antibody at less than or equal to 0.39 µg/mL has been found to inhibit by 50% the biological effects of 50 ng/mL mouse IL-15 (14-8151) in a CTLL2 proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.仅用于科研。不用于诊断过程。

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参考文献：

中和1.PloS oneIL-15 participates in the respiratory innate immune response to influenza virus infection."16-7154 was used in Blocking experiments to suggest that local IL-15 deposits in the lung airways regulate the coordinated innate and adaptive immune responses to influenza infection."AuthorsVerbist KC,Rose DL,Cole CJ,Field MB,Klonowski KD功能检测2.mAbsDiscovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis."16-7154 was used in Functional assays to identify and characterise a humanised antibody against IL-15."AuthorsVicari AP,Schoepfer AM,Meresse B,Goffin L,Léger O,Josserand S,Guégan N,Yousefi S,Straumann A,Cerf-Bensussan N,Simon HU,Chvatchko Y3.Cell researchAnticancer immunotherapy by CTLA-4 blockade: obligatory contribution of IL-2 receptors and negative prognostic impact of soluble CD25."16-7154 was used in in vivo experiments to provide the first immunologically relevant biomarker, namely elevated serum sCD25, that predicts resistance to CTLA-4 blockade in patients with melanoma."AuthorsHannani D,Vétizou M,Enot D,Rusakiewicz S,Chaput N,Klatzmann D,Desbois M,Jacquelot N,Vimond N,Chouaib S,Mateus C,Allison JP,Ribas A,Wolchok JD,Yuan J,Wong P,Postow M,Mackiewicz A,Mackiewicz J,Schadendorff D,Jaeger D,Zörnig I,Hassel J,Korman AJ,Bahjat K,Maio M,Calabro L,Teng MW,Smyth MJ,Eggermont A,Robert C,Kroemer G,Zitvogel L4. The Journal of experimental medicineAnti-CD3 therapy permits regulatory T cells to surmount T cell receptor-specified peripheral niche constraints."16-7154 was used in Functional assays to investigate the involvement of regulatory T cells in anti-CD3 treatment for autoimmune diabetes."AuthorsNishio J,Feuerer M,Wong J,Mathis D,Benoist C

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