IL-17A Monoclonal Antibody (eBioMM17F3), Functional Grade, eBioscience™/IL-17A 单克隆 (eBioMM17F3), 功能抗体, eBioscience™

货号：16-7173-81,16-7173-85,16-7173-38

规格：50 µg,500 µg,5 mg

价格：1196,3618,24836

产品类型：流式抗体

品牌：eBioscience

抗原：IL-17A

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioMM17F3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The eBioMM17F3 antibody is a mouse IgG1 immunoglobulin which reacts with mouse IL-17A. The eBioMM17F3 antibody is a neutralizing antibody. The eBioMM17F3 antibody has been tested by indirect ELISA against mouse IL-17B, IL-17C, IL-17D, IL-17E, and IL-17F, and has been found not to react to these other IL-17 family members. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Applications Reported: The eBioMM17F3 antibody has been reported useful for neutralization of mouse IL-17A bioactivity and for intracellular staining of IL-17A-producing cells (IC Flow).Applications Tested: The Functional Grade Purified eBioMM17F3 antibody has been tested by LAL assay to verify low endotoxin level and has been tested for neutralization of IL-17A bioactivity.For in vitro neutralization, the Functional Grade purified eBioMM17F3 antibody at 8.0 ng/mL has been found to neutralize by 50% the biological effect of 5.0 ng/mL mouse IL-17A in an assay of IL-6 secretion by NIH/3T3 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).仅用于科研。不用于诊断过程。

数据

IL-17A Antibody (16-7173-81)Scientific reports 2016 -Figure 4 Cytokine responses and CD4 + T cell polarization of splenocytes. One week following the last booster, the splenocytes from vaccinated mice (n = 6) were incubated with corresponding antigen proteins (10 mug/mL) for 5 days. The supernatants were harvested, and the cytokine levels of ( a ) IFN-gamma, ( b ) IL-17A were determined. The means +- SEM were shown. Empty boxes representative without stimulation, black boxes representative splenocytes stimulate with corresponding proteins. Asterisks indicate significant differences. ( * P < 0.05, ** P < 0.01). ( c ) After 5 days incubation, the splenocytes were stimulated with PMA/ionomycin and Golgistop for 6 h. Cells were stained for CD3, CD4, IFN-gamma, or IL-17, and was analysed by flow cytometry. The responses of Th1 ( d ) and Th17 ( e ) of the AlPO 4 group and recombinant vaccine immunised groups were presented as column bar graph and shown as means +- SEM. Results represent three independent experiments ( * P < 0.05, ** P < 0.01, *** P < 0.001).

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参考文献：

流式1.Scientific reportsProtective efficacy of the chimeric Staphylococcus aureus vaccine candidate IC in sepsis and pneumonia models."16-7173 was used in Neutralization experiments to design a novel chimeric vaccine for S. aureus infection."ELISA2Infection and immunityT-bet regulates immunity to Francisella tularensis live vaccine strain infection, particularly in lungs."16-7173 was used in an ELISA assay to demonstrate that T-bet is important in host resistance to primary live vaccine strain infection and intranasal secondary challenge."AuthorsMelillo AA,Foreman O,Bosio CM,Elkins KL中和3.EMBO reportsNDR1 protein kinase promotes IL-17- and TNF-α-mediated inflammation by competitively binding TRAF3."16-7173 was used in Neutralization experiments to study how IL-17 signal transduction is regulated, and the importance of this in numerous autoimmune disorders."AuthorsMa C,Lin W,Liu Z,Tang W,Gautam R,Li H,Qian Y,Huang H,Wang X4.Journal of immunology (Baltimore, Md. : 1950)Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome."16-7173 was used in Blocking experiments to study the role of adaptive immune cells in acute respiratory distress syndrome (ARDS), suggesting that IL-17A released by αβTh17 cells plays a significant role and could be a target for therapy."AuthorsLi JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D功能检测5.Brain, behavior, and immunityTh17-biased RORγt transgenic mice become susceptible to a viral model for multiple sclerosis."16-7173 was used in Neutralization experiments to suggest that a Th17-biased "gain-of-function" mutation could increase susceptibility to virus-mediated demyelinating diseases."AuthorsMartinez NE,Sato F,Kawai E,Omura S,Takahashi S,Yoh K,Tsunoda I6.Mediators of inflammationReduction of IL-17A might suppress the Th1 response and promote the Th2 response by boosting the function of Treg cells during silica-induced inflammatory response in vitro."16-7173 was used in Functional assays to study the role of IL-17A in the silica-induced inflammatory response."AuthorsTang W,Liu F,Chen Y,Song L,Dai W,Li C,Weng D,Chen J

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