CD282 (TLR2) Monoclonal Antibody (6C2), Functional Grade, eBioscience™/CD282 (TLR2) 单克隆 (6C2), 功能抗体, eBioscience™

货号：16-9021-81,16-9021-83, 16-9021-38,16-9021-025,16-9021-050

规格：50 µg,200 µg,5 mg,25 mg,50 mg

价格：1752,4444,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD282 (TLR2)

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：6C2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）；Assay-Dependent（对照）；Assay-Dependent（FN）

产品详细信息Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). Mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Twoof these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.Applications Reported: 6C2 has been reported for use in flow cytometric analysis. Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, cat. 14-0161).Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes or Raw264.7 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.仅用于科研。不用于诊断过程。

数据

CD282 (TLR2) Antibody (16-9021-81) in FlowStaining of Raw264.7 cells with 0.5 µg Rat IgG2b Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.5 µg Anti-Mouse CD282 (TLR2) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

流式1.Frontiers in pharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells."Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry"2.Proceedings of the National Academy of Sciences of the United States of AmericaC-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells."16-9021 was used in Flow cytometry/Cell sorting to reveal the modulatory role of the C-type lectin, DCIR, in tuberculosis-associated inflammation."AuthorsTroegeler A,Mercier I,Cougoule C,Pietretti D,Colom A,Duval C,Vu Manh TP,Capilla F,Poincloux R,Pingris K,Nigou J,Rademann J,Dalod M,Verreck FA,Al Saati T,Lugo-Villarino G,Lepenies B,Hudrisier D,Neyrolles O3.Cell reportsDectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways."16-9021 was used in Flow cytometry/Cell sorting to determine a link between Dectin-1 and the regulation of sterile inflammation and oncogenesis."AuthorsSeifert L,Deutsch M,Alothman S,Alqunaibit D,Werba G,Pansari M,Pergamo M,Ochi A,Torres-Hernandez A,Levie E,Tippens D,Greco SH,Tiwari S,Ly NNG,Eisenthal A,van Heerden E,Avanzi A,Barilla R,Zambirinis CP,Rendon M,Daley D,Pachter HL,Hajdu C,Miller G中和4.PLoS pathogensDectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum."16-9021 was used in Blocking experiments to investigate how H. capsulatum induces inflammasome activation and IL-1β production, showing that dectin-2 is a primary receptor for NLRP3 inflammasome activation."AuthorsChang TH,Huang JH,Lin HC,Chen WY,Lee YH,Hsu LC,Netea MG,Ting JP,Wu-Hsieh BAIF5.The Journal of experimental medicineIncreased endothelial expression of Toll-like receptor 2 at sites of disturbed blood flow exacerbates early atherogenic events.

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