| Description:The HIT8a monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The HIT8a antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT8a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
CD8a Antibody (12-0089-41) in FlowNormal human peripheral blood cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or CD8a Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis. |
| 1.Journal of immunology (Baltimore, Md. : 1950)Human γδ thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling."12-0089 was used in Flow cytometry/Cell sorting to study how ex vivo-isolated γδ thymocytes produced negligible IFN-γ and lacked cytolytic activity against leukemia cells."AuthorsRibot JC,Ribeiro ST,Correia DV,Sousa AE,Silva-Santos B2.OncotargetCD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+tumor cells, and extends the survival of tumor-bearing humanized mice."12-0089 was used in Flow cytometry/Cell sorting to suggest that the CD3xPDL1 BiTE may be efficacious for patients with PDL1(+) solid tumors, in combination with other immunotherapies that do not specifically neutralize PD1 pathway-mediated immune suppression."AuthorsHorn LA,Ciavattone NG,Atkinson R,Woldergerima N,Wolf J,Clements VK,Sinha P,Poudel M,Ostrand-Rosenberg S3.British journal of cancerPD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients."12-0089 was used in Flow cytometry/Cell sorting to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients."AuthorsWu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S4.Journal of immunology (Baltimore, Md. : 1950)Human γδ thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling."12-0089 was used in Flow cytometry/Cell sorting to study how ex vivo-isolated γδ thymocytes produced negligible IFN-γ and lacked cytolytic activity against leukemia cells."AuthorsRibot JC,Ribeiro ST,Correia DV,Sousa AE,Silva-Santos B |
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