CD8a Monoclonal Antibody (RPA-T8), APC, eBioscience™

货号：17-0088-41,17-0088-42

规格：25tests,100tests

价格：1215,2629

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：RPA-T8

荧光染料：APC

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.06 µg)/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: The RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (17-0088-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0037-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD8a APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Critical care (London, England)Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients."17-0088 was used in Flow cytometry/Cell sorting to demonstrate that lymphocyte apoptosis is markedly increased in septic shock patients, and suggest an essential role for the PD-1/PD-L1 pathway in sepsis-induced immunosuppression."2.Journal of immunology (Baltimore, Md. : 1950)The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects."17-0088 was used in Flow cytometry/Cell sorting to study the role of LAG-3 on T cell functionality during persistent HIV-1 infection."AuthorsTian X,Zhang A,Qiu C,Wang W,Yang Y,Qiu C,Liu A,Zhu L,Yuan S,Hu H,Wang W,Wei Q,Zhang X,Xu J3.Journal of immunology (Baltimore, Md. : 1950)Assessment of immune interference, antagonism, and diversion following human immunization with biallelic blood-stage malaria viral-vectored vaccines and controlled malaria infection."17-0088 was used in Flow cytometry/Cell sorting to study the effects of both controlled malaria infection, and human immunisation with biallelic blood-stage malaria viral-vectored vaccines, on the immune system."AuthorsElias SC,Collins KA,Halstead FD,Choudhary P,Bliss CM,Ewer KJ,Sheehy SH,Duncan CJ,Biswas S,Hill AV,Draper SJ

技术参数

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