| 抗体类型 | 单抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | 1 μg/test (Flow)； |
| 产品详细信息Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays (Please use Functional Grade purified RPA-T4, cat. 16-0049, in functional assays.).Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (14-0049-80) in FlowStaining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD4 Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Respiratory researchExpression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung."14-0049 was used in Immunohistochemistry to identify which adhesion molecules recruit specific subsets of lymphocytes into human bronchus-associated lymphoid tissue."AuthorsKawamata N,Xu B,Nishijima H,Aoyama K,Kusumoto M,Takeuchi T,Tei C,Michie SA,Matsuyama T2.OncotargetCD4+ and CD8+ T cells have opposing roles in breast cancer progression and outcome."14-0049 was used in Immunohistochemistry to delineate the dynamics and roles of CD4+ and CD8+ T cells in the pathogenesis of breast cancer."AuthorsHuang Y,Ma C,Zhang Q,Ye J,Wang F,Zhang Y,Hunborg P,Varvares MA,Hoft DF,Hsueh EC,Peng G3.Proceedings of the National Academy of Sciences of the United States of AmericaRemarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions."Published figure using CD4 monoclonal antibody (Product # 14-0049-82) in Flow Cytometry"4.eLifeThe HIV-1 envelope protein gp120 is captured and displayed for B cell recognition by SIGN-R1(+) lymph node macrophages."14-0049 was used in Immunohistochemistry to reveal a specialised lymph node antigen delivery system capable of delivering gp120 and other pathogen-derived glycoproteins to B cells."5.BMC musculoskeletal disordersCannabinoid receptor 2 as a potential therapeutic target in rheumatoid arthritis."14-0049 was used in Immunofluorescence to suggest that a selective CB(2) agonist could be a new therapy for rheumatoid arthritis that inhibits production of inflammatory mediators from FLS, and osteoclastogenesis."AuthorsFukuda S,Kohsaka H,Takayasu A,Yokoyama W,Miyabe C,Miyabe Y,Harigai M,Miyasaka N,Nanki T |
QQ:1749072012