| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.06 μg)/test (Flow) |
| 产品详细信息Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (47-0047-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD4 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Annals of the rheumatic diseasesIL-6-driven STAT signalling in circulating CD4+ lymphocytes is a marker for early anticitrullinated peptide antibody-negative rheumatoid arthritis."47-0047 was used in Flow cytometry/Cell sorting to investigate IL-6 mediated STAT3 signalling in circulating lymphocytes to better understand rheumatoid arthritis pathogenesis, and identify potential clinical biomarkers."AuthorsAnderson AE,Pratt AG,Sedhom MA,Doran JP,Routledge C,Hargreaves B,Brown PM,Lê Cao KA,Isaacs JD,Thomas R2.PloS oneThe contribution of non-conventional T cells and NK cells in the mycobacterial-specific IFNγ response in Bacille Calmette-Guérin (BCG)-immunized infants."47-0047 was used in Flow cytometry/Cell sorting to suggest that unconventional T cells, such as Vδ2 γδ T cells, play a more prominent role in mycobacterial immune responses than was previously thought."AuthorsZufferey C,Germano S,Dutta B,Ritz N,Curtis N3.Clinical and experimental immunologyTolerogenic dendritic cells generated with dexamethasone and vitamin D3 regulate rheumatoid arthritis CD4+T cells partly via transforming growth factor-β1."47-0047 was used in Flow cytometry/Cell sorting to establish a method to generate stable tolDC by pharmacological modulation of human monocyte-derived DC."AuthorsAnderson AE,Swan DJ,Wong OY,Buck M,Eltherington O,Harry RA,Patterson AM,Pratt AG,Reynolds G,Doran JP,Kirby JA,Isaacs JD,Hilkens CM4.International journal of immunopathology and pharmacologyCharacteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease."Published figure using CD4 monoclonal antibody (Product # 47-0047-42) in Flow Cytometry"5.Scientific reportsCIB1 and CIB2 are HIV-1 helper factors involved in viral entry."Published figure using CD4 monoclonal antibody (Product # 47-0047-42) in Flow Cytometry" |
QQ:1749072012