FOXP3 Monoclonal Antibody (PCH101), eFluor 450, eBioscience™

货号：48-4776-42,48-4776-41

规格：100Tests,25 Tests

价格：3360,1888

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：PCH101

荧光染料：eFluor 450

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test

产品详细信息Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is amember of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and by flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (48-4776-42) in FlowSurface staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) (left) or Anti-Human CD25 APC (Product # 17-0259-42) (right) followed by fixation and permeabilization with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and intracellular staining with Anti-Human Foxp3 eFluor® 450. Cells in the lymphocyte gate were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.BloodMucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.AuthorsEpple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T2.PloS oneProtection against bronchiolitis obliterans syndrome is associated with allograft CCR7+ CD45RA- T regulatory cells."48-4776 was used in Flow cytometry/Cell sorting to hypothesize that frequencies of Treg in bronchoalveolar lavage fluid after lung transplantation would predict subsequent development of bronchiolitis obliterans syndrome."AuthorsGregson AL,Hoji A,Palchevskiy V,Hu S,Weigt SS,Liao E,Derhovanessian A,Saggar R,Song S,Elashoff R,Yang OO,Belperio JA3.Frontiers in immunologyAn Autocrine TNFα-Tumor Necrosis Factor Receptor 2 Loop Promotes Epigenetic Effects Inducing Human Treg StabilityIn Vitro."48-4776 was used in Flow Cytometry to explore the underlying mechanisms of rapamycin and tumour necrosis factor receptor 2 agonist-mediated regulatory T cell stability."AuthorsUrbano PCM,Koenen HJPM,Joosten I,He X4.Scientific reportsNiraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models."48-4776 was used in Flow cytometry/Cell sorting to investigate the therapeutic potential of combining niraparib with anti-P-1 immune checkpoint inhibitors in preclinical tumour models."AuthorsWang Z,Sun K,Xiao Y,Feng B,Mikule K,Ma X,Feng N,Vellano CP,Federico L,Marszalek JR,Mills GB,Hanke J,Ramaswamy S,Wang J5.Journal of immunology researchDNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4+CD25highFOXP3+Regulatory T Cells but Does Not Affect Their Suppressive Capacity."48-4776 was used in Flow cytometry/Cell sorting to examine the in vitro effect of derivative 5-aza-2'-deoxycytidine on the stability and function of FACS-sorted human naturally occurring CD4+CD25high FOXP3+ regulatory T cells."AuthorsLandman S,Cruijsen M,Urbano PCM,Huls G,van Erp PEJ,van Rijssen E,Joosten I,Koenen HJPM

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