CD154 (CD40 Ligand) Monoclonal Antibody (24-31), eBioscience™

货号：14-1548-80,14-1548-82

规格：25μg,100μg

价格：985,2193

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD154

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：24-31

荧光染料：其它

抗体类型:	流式抗体	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	1 µg/test (Flow）

产品详细信息Description:The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activationand induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: 24-31 has been reported for use in flow cytometric analysis. 24-31 has also been reported to block CD154 binding to CD40 and inhibit T cell-dependent B cell differentiation and antibody response in vitro . (Please use Functional Grade Purified 24-31, cat. 16-1548, in functional assays.).Applications Tested: The 24-31 antibody has been tested by flow cytometric analysis of resting and activated human normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD154 (CD40 Ligand) Antibody (14-1548-80)Oncology letters 2018 -Figure 1. The remnants of LM8 decreased the co-stimulatory molecules, and inhibited IL-12 and IL-6 levels, increased IL-10 levels, and inhibited the ability of DCs to stimulate T-cell proliferation. (A) The DCs were isolated following a 48-h co-culture with the remnants of LM8 cells. The cells were then labeled with antibodies against CD11c, MHC-2, CD40, CD86, CD80 and CCR7 for phenotypic analysis by flow cytometry. The numbers in the histograms indicate the geometric mean fluorescence intensity. (B) Following isolation from the co-culture system, the DCs were cultured for 12 h. Subsequently, the expression levels of IL-12, IL-6 and IL-10 in the supernatant were analyzed by ELISA. (C) CD4 + T cells from DO11.10 OVA 323-339 -specific (TCR-transgenic x C57BL/6) F1 hybrid mice were co-cultured with DCs or mDCs (control) in the presence of OVA peptides. Five days later, the total number of viable CD4 + T (CD4 + 7-AAD - ) cells in each well was measured by flow cytometry. Data represent one of at least three experiments with similar results. *P<0.05 LM8 DC/T vs. Control DC/T. IL, interleukin; DCs, dendritic cells; CD, cluster of differentiation; MHC-2, major histocompatibility complex 2; CCR7, C-C chemokine receptor 7; DC/T, DCs and T cell co-culture; mDCs, mature DCs.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.Journal of virologyCD4-like immunological function by CD4- T cells in multiple natural hosts of simian immunodeficiency virus."14-1548 was used in Flow cytometry/Cell sorting to demonstrate that coevolution with SIV has, in several cases, involved downregulation of receptors for the virus by otherwise-susceptible host target cells."2.Cytometry. Part A : the journal of the International Society for Analytical CytologyOptimization of mass cytometry sample cryopreservation after staining."14154882 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment"3.Journal of virologyCD4-like immunological function by CD4- T cells in multiple natural hosts of simian immunodeficiency virus."14-1548 was used in Flow cytometry/Cell sorting to demonstrate that coevolution with SIV has, in several cases, involved downregulation of receptors for the virus by otherwise-susceptible host target cells."4.Leukemia researchComparative analysis of MVA-CD40L and MVA-TRICOM vectors for enhancing the immunogenicity of chronic lymphocytic leukemia (CLL) cells."14-1548 was used in Blocking experiments to compare the immunogenicity-enhancing properties of modified vaccinia virus strain Ankara encoding either CD40L or LFA-3 (TRICOM)."

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-27285.htm