CD45RB Monoclonal Antibody (PD7/26)

货号：14-9458-82,14-9458-37

规格：100 ug,2 mg

价格：1260,11562

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RB

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：PD7/26

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	5 µg/mL(WB)；5 µg/mL(IHC (P))；Assay-Dependent(ICC)；Assay-Dependent(IF)；5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The monoclonal antibody PD7/26 recognizes CD45RB, which is an isoform of the Leukocyte Common Antigen (LCA) also known as CD45. CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. Expression of CD45RB is more restricted than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been show to express low levels of CD45RB, which may correlate with increasedmigration to sites of infection. In contrast, high CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro.Applications Reported: This PD7/26 antibody has been reported for use in Flow Cytometric Analysis, Western Blotting, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, and Immunocytochemistry.Applications Tested: This PD7/26 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil using low pH antigen retrieval. This can be used at less than or equal to 5 µg/mL. This PD7/26 antibody has also been tested by western blot at 5 µg/mL and and by flow cytometric analysis on human peripheral blood cells at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

数据

CD45RB Antibody (14-9458-82) in IHC (P)Immunohistochemistry on formalin-fixed paraffin embedded human tonsil, using 5 µg/mL of Mouse IgG1 Isotype Control (left) or 5 µg/mL Anti-Human CD45RB Purified (right) followed by Anti-Mouse IgG Biotin, and DAB visualization.Nuclei are counterstained with hematoxylin.

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