CD45RO Monoclonal Antibody (UCHL1)

货号：14-0457-82,14-0457-37,14-0457-95

规格：100 ug,2 mg,10 mg

价格：1260,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD45RO

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：UCHL1

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.Applications Tested: The UCHL1 antibodyhas been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

数据

CD45RO Antibody (14-0457-82) in FlowStaining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human CD45RO Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells.2. Respiratory researchExpression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.3. Cell researchBlocking the recruitment of naive CD4+T cells reverses immunosuppression in breast cancer.4. OncotargetEfficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.5. The EMBO journalTET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.

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