| 产品介绍 | | |
| The Magic Red Caspase-3/7 kituses a quick and easy method to analyze intracellular active caspases in apoptotic cells. |
| 产品详情 | | |
| Product Form | Pack Size: 25 TestsMagic Red Substrate MR-(DEVD)2, 25 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mLPack Size: 100 TestsMagic Red Substrate MR-(DEVD)2, 100 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mL |
| Test Principle | Caspase Magic Red kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red reagent contains a caspase inhibitor sequence (DEVD)2linked to a red (Cresyl Violet) fluorescent probe.The Caspase Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates. |
| Approx. Protein Concentrations | Magic Red™ is a trademark of Immunochemistry Technologies, LLC. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Store the unopened kit and each unopened component at +4℃ until the expiration date.Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20℃ for 6 months protected from light and thawed no more than twice during that time. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunofluorescence | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 产品推荐 |
| Description | Product Code | Applications | Pack Size |
| Magic Red™ Caspase-3/7 Kit | ICT936 | IF | 100 Tests |
| 文献 |
| 1.Wei, Z.et al.(2011) Notch activation enhances the microglia-mediated inflammatory response associated with focal cerebral ischemia.Stroke. 42 (9): 2589-94.2.Shibata, Y.et al.(2016) Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL methodApplied Spectroscopy Reviews. Mar 13 [Epub ahead of print]3.McHugh, B.J.et al.(2019) Cathelicidin is a "fire alarm", generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection withPseudomonas aeruginosa..PLoS Pathog. 15 (4): e1007694. |
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