| 产品介绍 | | |
| Block Ace is designed as a high-performance blocking reagent for use in immunological assays such as ELISA and Western blotting. It may also be used for dilution of antibodies and for washing procedures in the above assays.Block Ace has been shown to have superior performance to 1% BSA in blocking in ELISA assays. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves.For blocking in ELISA applications, we recommend using a 1/4 dilution of the reconstituted solution.For blocking in Western Blotting applications, we recommend using the reconstituted solution neat.For washing applications we recommend a 1/10 dilution of the reconstituted solution, and adding Tween 20 to a level of 0.05-0.2% v/v.For use as a test sample or secondary antibody diluent we recommend a 1/10 dilution of the reconstituted solution. |
| 产品详情 | | |
| Reconstitution | Reconstitute each 4g sachet in 100ml distilled water. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed for 1 week from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 1/4 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Yamashita, M.et al.(2010) Morphological and extracellular matrix changes following vocal fold injury in mice.Cells Tissues Organs. 192 (4): 262-71.2.Head, E.et al.(2010) Amyloid-beta peptide and oligomers in the brain and cerebrospinal fluid of aged canines.J Alzheimers Dis. 2010;20(2):637-46.3.Hara, H.et al.(1990) Enzyme immunoassay for measuring antibodies against skeletal muscle in patients with myasthenia gravis.Clin Chem. 36 (11): 1967-9. |
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