| 产品介绍 | | |
| Rabbit anti Human ORAI3 antibodyrecognizes the N-terminal region of human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family.Antigen stimulation of immune cells triggers Ca2+entry through Ca2+release-activated Ca2+(CRAC) channels. CRAC channels are the main pathway for Ca2+influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+sensor. ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.Rabbit anti Human ORAI3 antibody (AHP1496) is not expected to cross react with either ORAI1 or ORAI2. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Antiserum Preparation | Antiserum to human ORAI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.02% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Immunogen | A 15 amino acid peptide from near the amino terminus of human ORAI3. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Western Blotting | √ | 1 | 2ug/ml |
Immunohistology - Frozen | √ | | 10ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Western Blotting
- AHP1496 detects a band of approximately 35kDa in A20 cell lysates.
|
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
Immunocytochemical staining of A20 cells with Rabbit anti Human ORAI3 (AHP1496) |
| 文献 |
| 1. Feske, S.,et al. (2006) A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.Nature; 441:179-852. Soboloff, J.et al.(2006) Calcium signals mediated by STIM and Orai proteins--a new paradigm in inter-organelle communication.Biochim Biophys Acta. 1763 (11): 1161-8.3. Mercer, J.C.et al.(2006) Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1.J Biol Chem. 281 (34): 24979-90.4. Lewis, R.S. (2001) Calcium signaling mechanisms in T lymphocytes.Annu Rev Immunol. 19: 497-521. |
QQ:1749072012